Du sökte efter: Elektroforesreagens

Leverantör: Rockland Immunochemicals

Beskrivning: These Chemiluminescent Western Blot Kits allow for the detection of primary polyclonal or monoclonal antibodies provided by the user. After protein separation and transfer, the membrane is probed with primary antibody. Detection of the membrane-bound primary antibody-antigen complex is achieved by the addition of a secondary antibody conjugated to the enzyme horseradish peroxidase. The enzyme reacts with a specialised formulation of luminol, an extremely sensitive, non radioactive substrate that emits light and allows visualisation using X-ray film or other imaging methods, including highly sensitive CCD cameras and imaging systems. Because of the extremely high sensitivity of the kit, primary and secondary antibodies can be used at greater dilutions. Primary antibodies currently diluted 1:1000 using an ECL™ substrate is the equivalent of a 1:5000 dilution using the FemtoMax™ substrate.

Artikel nummer : (TB110)

Leverantör: G-Biosciences

Beskrivning: Kingdom blot is prepared using total protein extracted from a variety of species, where whole species is not available the whole liver lysate is used. These blots can be used to identify similar proteins in other species, including human, chicken, frog, worm, Drosophila, yeast, Aspergillus, <i>E. coli</i>, Rhizobium and green algae.

UOM: 1 * 1 Items

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Leverantör: Cytiva

Beskrivning: The appearance of streaks that distort 2-D electrophoresis maps is a common problem, occurring most frequently when running gels that contain regions greater than pH 7,0. Increased sample load, increased length of the IPG strip, or using a narrower pH gradient worsens the problem. Extra spots on 2-D gels, caused by non specific oxidation of proteins, is another difficulty encountered when running gels containing basic regions. Both streaking and non specific oxidation result in poorly resolved protein patterns and reduced reproducibility between electrophoresis runs.

Leverantör: Cytiva

Beskrivning: Med Rainbow™ molekylviktsmarkörer går det snabbare och enklare att identifiera proteiner på SDS-polyakrylamidgel. De laddningsklara markörerna ger skarpare och mer intensiva band på gel och blot och har förbättrade bandavstånd för mer exakt angivelse av molekylvikter. Markörernas klara och distinkta färger gör det enklare att bekräfta överföringen till blotting-membran och riktning.

Certifikat

Artikel nummer : (RPN3006)

Leverantör: Cytiva

Beskrivning: ECL Direct Nucleic Acid Labeling and Detection Systems are based on the direct labeling of DNA or RNA probes with horseradish peroxidase (HRP) in a simple 20 min chemical reaction. The resulting probe can be used without purification. Detection is achieved by generation of light via the HRP-catalyzed breakdown of luminol.

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Leverantör: Cytiva

Beskrivning: AlkPhos Direct™ Labelling and detection systems are based on the rapid, direct labelling of DNA or RNA probes with thermostable alkaline phosphatase. AlkPhos Direct™ combines the convenience of direct enzyme labelling (no blocking or antibody stages) with those of alkaline phosphatase detection (long light output and high sensitivity).

Leverantör: Cytiva

Beskrivning: CypHer5E is a red-excitable, pH-sensitive cyanine dye derivative that is minimally fluorescent at a basic pH and maximally fluorescent at an acidic pH.

Artikel nummer : (RPN2235)

Leverantör: Cytiva

Beskrivning: Amersham ECL Select is a high sensitivity reagent for chemiluminescent Western blotting detection.

UOM: 1 * 1 KIT

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Leverantör: Cytiva

Beskrivning: Enhanced chemiluminescence (ECL) detection reagents for reliable detection of medium and high expression proteins.

Säkerhetsdatablad Certifikat

Leverantör: Cytiva

Beskrivning: Cy™ fluorescent amidites for incorporation of Cy3, Cy3,5, Cy5, Cy5,5 fluorescent dyes at any position in an oligonucleotide during synthesis.

Leverantör: Cytiva

Beskrivning: Chemiluminescence detection reagent for detection of high to medium abundant proteins.

Artikel nummer : (RPN3005)

Leverantör: Cytiva

Beskrivning: ECL Direct Nucleic Acid Labeling and Detection Systems are based on the direct labeling of DNA or RNA probes with horseradish peroxidase (HRP) in a simple 20 minute chemical reaction. The resulting probe can be used without purification. Detection is achieved by generation of light via the HRP-catalyzed breakdown of luminol.

UOM: 1 * 1 Items

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Artikel nummer : (RPN5785)

Leverantör: Cytiva

Beskrivning: ECF™ substrate for Western blotting

UOM: 1 * 1 Items

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Leverantör: Cytiva

Beskrivning: pI markers are lyophilized mixtures of stable, salt-free, highly purified proteins.

Leverantör: Cytiva

Beskrivning: Pharmalyte® carrier ampholytes, prepared by the co-polymerization of glycine, glycylglycine, amines and epichlorohydrin, are available in five broad-range and four narrow-range pH intervals.

Säkerhetsdatablad Certifikat

Leverantör: Cytiva

Beskrivning: Amersham ECL™ Prime är en mycket känslig detektionsreagens för Western blotting som kännetecknas av stabil signalemission och som möjliggör upprepade exponeringar och gör det enklare att behandla flera blottar i samma experimentella körning. Amersham ECL™ Prime ger ett detektionssystem som är känsligt, stabilt och ger exakta kvantitativa resultat för ett stort dynamiskt område av proteinnivåer och som har en snål förbrukning av dyra antikroppsreagenser. Den höga prestandan hos Amersham ECL™ Prime beror på att det finns en förstärkare i reagensen som ökar enzymomsättningen och därmed ökar både signalintensiteten och varaktigheten avsevärt. Ljussignalen förstärks ytterligare om man lägger till en katalysator.