Chemiluminescent western blot kits, FemtoMax™

Leverantör: Rockland Immunochemicals

KCA001 KCA002 KCA003 KCA004
ROCKKCA001EA 6400 SEK
ROCKKCA001 ROCKKCA002 ROCKKCA003 ROCKKCA004
Chemiluminescent western blot kits, FemtoMax™
Elektroforesreagens Western Blotting Reagents

These Chemiluminescent Western Blot Kits allow for the detection of primary polyclonal or monoclonal antibodies provided by the user. After protein separation and transfer, the membrane is probed with primary antibody. Detection of the membrane-bound primary antibody-antigen complex is achieved by the addition of a secondary antibody conjugated to the enzyme horseradish peroxidase. The enzyme reacts with a specialised formulation of luminol, an extremely sensitive, non radioactive substrate that emits light and allows visualisation using X-ray film or other imaging methods, including highly sensitive CCD cameras and imaging systems. Because of the extremely high sensitivity of the kit, primary and secondary antibodies can be used at greater dilutions. Primary antibodies currently diluted 1:1000 using an ECL™ substrate is the equivalent of a 1:5000 dilution using the FemtoMax™ substrate.


Observera: Wash buffers MUST NOT contain SODIUM AZIDE or other inhibitors of peroxidase activity!

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