Du sökte efter: Merck Millipore (Novagen)

Artikel nummer : (71546-3)

Leverantör: Merck Millipore (Novagen)

Beskrivning: The Novagen® BacMagic™-1 transfection kit provides a positive selection for creating baculovirus recombinants in preparation for recombinant protein expression in insect cells. The kit improves on the traditional method for generating recombinant baculoviruses by eliminating the tedious, time-consuming steps of plaque purification.

UOM: 1 * 1 KIT

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Leverantör: Merck Millipore (Novagen)

Beskrivning: NovaBlue competent cells are designed for convenient and reliable plasmid transformation. NovaBlue is a K-12 strain ideally suited as an initial cloning host due to its high transformation efficiency, blue/white screening capability (with appropriate plasmids), and <i>recA</i> and <i>endA</i> mutations, which result in high yields of excellent-quality plasmid DNA. Standard NovaBlue and convenient NovaBlue Singles™ are ideal for routine cloning, while NovaBlue GigaSingles™ are most suitable for applications requiring higher transformation efficiencies.

Artikel nummer : (71004-3)

Leverantör: Merck Millipore (Novagen)

Beskrivning: The 10 mM dNTP mix is a ready-to-use preparation of ultrapure dATP, dCTP, dGTP, and dTTP (monosodium salts) at a concentration of 10 mm each in sterile deionised water at pH 7,0. Qualified for any application that requires pure deoxynucleotides, such as PCR, cDNA synthesis, and fill-in reactions.

UOM: 1 * 0,2 mL

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Leverantör: Merck Millipore (Novagen)

Beskrivning: Pellet Paint® Co-Precipitant är en synlig, infärgad carriermolekyl speciellt utarbetad för användning vid alkoholutfällning av nukleinsyror. Reaktionen tar 2 minuter och kräver ej inkubation vid låg temperatur eller förlängd centrifugering. Både RNA och DNA utfälls effektivt, även i de mest utspädda lösningar (2 ng/ml) och pelleten med dess rosa färg spåras enkelt. Pelleten kan enkelt följas under tvättstegen, vilket förhindrar förluster under hantering och säkerställer att pelleten resuspenderas.

Leverantör: Merck Millipore (Novagen)

Beskrivning: A common method for transformation of plasmids into E.coli is the use of chemically competent cells. Although competent cells can be prepared in the laboratory, greater efficiency, reproducibility, and convenience are achieved using Novagen® prepared competent cells. Every Novagen® competent cell strain is verified for phenotype and purity, and is guaranteed for transformation efficiency.

Artikel nummer : (70976-3)

Leverantör: Merck Millipore (Novagen)

Beskrivning: The SpinPrep™ PCR Clean Up Kit is designed for the rapid purification of DNA amplified by PCR. The 10-minute procedure involves addition of a binding buffer followed by adsorption of the DNA to a silica membrane in a spin column format. Following a wash step, the DNA is eluted in low salt buffer. This kit removes DNA polymerises, dNTPs, salts, and >99% of primers so that they do not interfere with downstream applications such as cloning, sequencing, or labelling. PCR products from 100 to >12000 bp can be cleaned up, with recoveries of amplified DNA in the range of 60 to 90% under standard conditions.

UOM: 1 * 100 Tests

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Artikel nummer : (69022-3)

Leverantör: Merck Millipore (Novagen)

Beskrivning: The thrombin cleavage capture kit is designed for cleavage of fusion proteins followed by convenient and quantitative removal of thrombin protease. The method is based on the use of biotinylated thrombin for digestion and its subsequent removal with streptavidin agarose.

UOM: 1 * 1 KIT

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Leverantör: Merck Millipore (Novagen)

Beskrivning: BugBuster® Protein Extraction-reagens är formulerad för varsam fraktionering av cellväggar från<i> E. coli</i>, vilket frigör lösligt protein. Detta ger ett enkelt, snabbt och billigt alternativ till mekaniska metoder som French Press eller sonikering. Formulan använder en detergentmix som är utformad för att perforera cellväggar utan att denaturera lösligt protein.

Artikel nummer : (71285-3)

Leverantör: Merck Millipore (Novagen)

Beskrivning: BCA Protein Assay is a simple and reliable protein quantification method. It can be used to determine protein concentration in the range of 20-2000 µg/ml in either a standard assay or microassay configuration.

UOM: 1 * 1 KIT

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Leverantör: Merck Millipore (Novagen)

Beskrivning: Ni-NTA HisBind® Superflow™ Resin is a medium-pressure compatible version of the Ni-NTA HisBind® Resin

Leverantör: Merck Millipore (Novagen)

Beskrivning: pET-41 series is designed for cloning and high-level expression of peptide sequences fused with the 220 aa GST•Tag™ protein. The cloning/expression region of the coding strand transcribed by T7 RNA polymerase. The f1 origin is oriented so that infection with helper phage will produce virions containing single stranded DNA that corresponds to the coding strand.

Artikel nummer : (69909-3)

Leverantör: Merck Millipore (Novagen)

Beskrivning: The pET-30a-c(+) vectors carry an N-terminal His Tag®/thrombin/S Tag™/enterokinase configuration plus an optional C-terminal His tag sequence.

UOM: 1 * 10 µG

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Leverantör: Merck Millipore (Novagen)

Beskrivning: PopCulture® Reagent is a detergent-based concentrate that can be added directly to cultures of <i>E. coli</i> to effectively extract proteins without the need for cell harvest. Recombinant proteins can be directly screened in the crude extract, or purified by adding an affinity matrix, washing the matrix-target protein complex to remove spent culture medium and cellular contaminants, and eluting the purified protein from the matrix. The PopCulture protein purification procedure is ideally suited to high-throughput robotic processing of samples for proteomics research or any application that would benefit from the increased speed and convenience.

Leverantör: Merck Millipore (Novagen)

Beskrivning: A common method for transformation of plasmids into E.coli is the use of chemically competent cells. Although competent cells can be prepared in the laboratory, greater efficiency, reproducibility, and convenience are achieved using Novagen® prepared competent cells. Every Novagen® competent cell strain is verified for phenotype and purity, and is guaranteed for transformation efficiency.

Artikel nummer : (70793-3)

Leverantör: Merck Millipore (Novagen)

Beskrivning: BugBuster®Ni-NTA HIS-BIND® Purification Kit is used for protein purification. Ni-NTA HIS-BIND® Resin is high-performance Ni2+-charged agarose used for rapid, one-step purification of proteins containing a polyhistidine tag sequence.

UOM: 1 * 1 KIT

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Leverantör: Merck Millipore (Novagen)

Beskrivning: The Overnight Express™ Autoinduction Systems enable regulated protein expression in <i>E. coli</i>, without monitoring the culture or adding inducer during cell growth.