Du sökte efter: Merck Millipore (Novagen)

Artikel nummer : (71978-3)

Leverantör: Merck Millipore (Novagen)

Beskrivning: The One Step RT-PCR Master Mix Kit allows rapid, sensitive analysis of gene expression from tissues and cells. It can replace methods for detecting and quantifying gene expression such as Northern blots,<i> in situ</i> hybridisation, dot blots, S nuclease assays and conventional two step RT-PCR. The kit utilises recombinant <i>Thermus thermophilus</i> (rTth) DNA Polymerase, which acts as both a thermostable RNA dependent DNA polymerase and a DNA dependent DNA polymerase. The rTth DNA Polymerase is provided in a 2X master mix with an antibody for antibody-mediated hot start, optimised buffer, and ultrapure deoxynucleotides. Antibody-mediated Hot Start enhances specificity of both reverse transcription and PCR. The kit enables cDNA synthesis from input RNA followed by PCR amplification of the cDNA in a single reaction, with no additional hands-on requirement for buffer changes or adding reagents. Typically, detection of a specific transcript requires only 2 hours.

UOM: 1 * 1 KIT

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Artikel nummer : (71840-3)

Leverantör: Merck Millipore (Novagen)

Beskrivning: The HisTag® Antibody HRP Conjugate kit contains a horseradish peroxidase-conjugated HisTag® Monoclonal Antibody that is useful as a single detection reagent, thus eliminating cross-reactivity associated with secondary antibody reagents. It recognises five consecutive histidine residues regardless of the surrounding amino acid context. The purified antibody conjugate produces a strong signal at a 1:1000 to 1:2000 dilution on Western blots and dot blots. The HisTag® Antibody HRP Conjugate also detects the recombinant marker bands in the Novagen Perfect Protein™ Western Markers for convenient visualisation of accurate internal standards on Western blots.

UOM: 1 * 125 µl

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Leverantör: Merck Millipore (Novagen)

Beskrivning: GST Bind™ Resin utilizes an 14-atom spacer arm to covalently attach reduced glutathione via a sulfide linkage. The high degree of substitution of glutathione ensures a high binding capacity with yields of glutathione S-transferase (GST) fusion proteins of 5 to 8 mg/ml settled resin. The resin can be re-used up to 6 times without loss of capacity.

Leverantör: Merck Millipore (Novagen)

Beskrivning: Pellet Paint® NF-utfällningsmedlet är en bärare för icke-fluorescerande färgmärkning som är kompatibel med fluorescerande sekvensering. Det underlättar snabbt avlägsnande av BigDye®-terminatorer under alkoholutfällningen av reaktionsprodukter vid cykelsekvensering. Utfällning vid cykelsekvenseringsreaktioner sker snabbt med centrifugeringstider på 10 minuter. Den lätt visualiserade bäraren är en enkel bekräftelse på att utfällning har skett. Sekvenseringsreaktionsprodukter görs enkelt till pelletar, och färgmärkta terminatorer finns kvar i supernatantvätskan vid alkoholutfällning med standardprotokollen för utfällning från Applied Biosystems. Återsuspension av pelletprodukter från sekvenseringsreaktionen i avjoniserad formamid kan bekräftas genom att bärarpelleten kontrolleras för att se om den upplösts.

Leverantör: Merck Millipore (Novagen)

Beskrivning: The Overnight Express™ Autoinduction Systems enable regulated protein expression in <i>E. coli</i>, without monitoring the culture or adding inducer during cell growth.

Leverantör: Merck Millipore (Novagen)

Beskrivning: pET-41 series is designed for cloning and high-level expression of peptide sequences fused with the 220 aa GST•Tag™ protein. The cloning/expression region of the coding strand transcribed by T7 RNA polymerase. The f1 origin is oriented so that infection with helper phage will produce virions containing single stranded DNA that corresponds to the coding strand.

Leverantör: Merck Millipore (Novagen)

Beskrivning: PopCulture® Reagent is a detergent-based concentrate that can be added directly to cultures of <i>E. coli</i> to effectively extract proteins without the need for cell harvest. Recombinant proteins can be directly screened in the crude extract, or purified by adding an affinity matrix, washing the matrix-target protein complex to remove spent culture medium and cellular contaminants, and eluting the purified protein from the matrix. The PopCulture protein purification procedure is ideally suited to high-throughput robotic processing of samples for proteomics research or any application that would benefit from the increased speed and convenience.

Leverantör: Merck Millipore (Novagen)

Beskrivning: Ni-NTA HisBind® Superflow™ Resin is a medium-pressure compatible version of the Ni-NTA HisBind® Resin

Leverantör: Merck Millipore (Novagen)

Beskrivning: A common method for transformation of plasmids into E.coli is the use of chemically competent cells. Although competent cells can be prepared in the laboratory, greater efficiency, reproducibility, and convenience are achieved using Novagen® prepared competent cells. Every Novagen® competent cell strain is verified for phenotype and purity, and is guaranteed for transformation efficiency.

Artikel nummer : (70793-3)

Leverantör: Merck Millipore (Novagen)

Beskrivning: BugBuster®Ni-NTA HIS-BIND® Purification Kit is used for protein purification. Ni-NTA HIS-BIND® Resin is high-performance Ni2+-charged agarose used for rapid, one-step purification of proteins containing a polyhistidine tag sequence.

UOM: 1 * 1 KIT

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Artikel nummer : (69909-3)

Leverantör: Merck Millipore (Novagen)

Beskrivning: The pET-30a-c(+) vectors carry an N-terminal His Tag®/thrombin/S Tag™/enterokinase configuration plus an optional C-terminal His tag sequence.

UOM: 1 * 10 µG

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Leverantör: Merck Millipore (Novagen)

Beskrivning: A common method for transformation of plasmids into <i>E.coli</i> is the use of chemically competent cells. Although competent cells can be prepared in the laboratory, greater efficiency, reproducibility, and convenience are achieved using Novagen® prepared competent cells. Every Novagen® competent cell strain is verified for phenotype and purity, and is guaranteed for transformation efficiency.

Leverantör: Merck Millipore (Novagen)

Beskrivning: Novagen® T7Select® Phage Display System takes advantage of the properties of bacteriophage T7. This system is easy to use and has the capacity to display peptides up to about 50 amino acids in size in high copy number (415 per phage), and peptides or proteins up to about 1200 amino acids in low copy number (0,1 to 1 per phage) or mid-copy number (5 to 15 per phage).

Artikel nummer : (71366-4)

Leverantör: Merck Millipore (Novagen)

Beskrivning: The Overnight Express™ Autoinduction Systems enable regulated protein expression in <i>E. coli</i>, without monitoring the culture or adding inducer during cell growth.

UOM: 1 * 1 Items

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Leverantör: Merck Millipore (Novagen)

Beskrivning: Biotinylated Thrombin has covalently attached biotin for easy removal of the enzyme from cleavage reactions using immobilised streptavidin. This preparation is tested for activity using the same assay as for unmodified thrombin, and for >99% binding to streptavidin agarose.

Leverantör: Merck Millipore (Novagen)

Beskrivning: THP [Tris(hydroxypropyl)phosphine; C9H21O3P] is a water-soluble, odorless, sulfhydryl reducing agent that is more stable and effective than dithiothreitol (DTT).