Du sökte efter: Quantabio

Leverantör: Quantabio

Beskrivning: 5PRIME Phase Lock Gel (PLG) eliminerar proteinkontaminering mellan faserna under fenolextraktion och säkerställer snabbare resultat med förbättrat utbyte. PLG migrerar och bildar en tät förslutning mellan faserna i en vatten-/organfas under centrifugeringen. Den organiska fasen och ämnen mellan faserna fångas effektivt in i eller under barriären, vilket möjliggör enkel dekantering eller pipettering av hela vattenfasen. Fördelarna är ökat utbyte med upp till 30%, ökat skydd från exponering för farliga ämnen samt obefintlig risk för provkontamination mellan faserna. För din bekvämlighet levereras PLG färdigdispenserat i vanliga centrifugrör av varierande storlekar. PLG finns i två olika densitetsformuleringar - Heavy och Light - och kan användas i snart sagt alla protokoll som innefattar extraktion med fenol och/eller kloroform, genom att helt enkelt välja den formulering som lämpar sig för de organiska faserna och vattenfaserna.

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Leverantör: Quantabio

Beskrivning: qScript™ cDNA-synteskit innehåller qScript™, reverstranskriptas, en blandning av MMLV, reverstranskriptas, och ribonukleashämmande protein, som optimerats för känslig och tillförlitlig cDNA-syntes med ett brett dynamiskt omfång av införd RNA. cDNA-syntesreagens finns i flera olika supermixar och kit som passar specifika tillämpningar.

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Leverantör: Quantabio

Beskrivning: Q qPCR cycler uses a patented magnetic induction technology to rapidly heat samples, coupled with fan-forced air for cooling to acquire data in only 25 minutes. Available as four channel models, the robust optical system acquires all channels simultaneously and allows for running the fastest multiplexed assays.

Leverantör: Quantabio

Beskrivning: qScript™ XLT One-Step RT-qPCR ToughMix® is a ready to use, highly sensitive master mix for reverse transcription quantitative PCR (RT-qPCR) of RNA templates.

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Leverantör: Quantabio

Beskrivning: An optimised kit for the rapid construction of DNA libraries from fragmented double-stranded DNA for sequencing on Illumina® NGS platforms and prepares for sequencing success with the highest quality library.

Leverantör: Quantabio

Beskrivning: Ultra-Plex™ 1-Step ToughMix® is a ready-to-use, 4X-concentrated master mix for reverse transcription quantitative PCR (RT-qPCR) of RNA templates using hybridisation probe detection chemistries such as TaqMan® 5’-hydrolysis probes on Real-Time PCR systems that utilise ROX passive reference with 490 nM excitation. First-strand cDNA synthesis and PCR amplification are carried out in the same tube without opening between procedures. It is ideal for highly sensitive quantification of RNA viruses or low abundance RNA targets in uni- or multiplexed RT-qPCR applications as well as high throughput gene-expression studies. The system has been optimised to deliver maximum RT-qPCR efficiency, sensitivity, and specificity in reduced reaction volumes and fast cycle times. Ultra-Plex™ 1-Step ToughMix® contains all required components for RT-qPCR except RNA template and probe. It is compatible with all dual-labeled probe chemistries.

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Leverantör: Quantabio

Beskrivning: Superior sensitivity and multiplexing for DNA amplification of long targets.

Leverantör: Quantabio

Beskrivning: qScript™ One-Step SYBR® Green qRT-PCR Kits reliably deliver maximum qRT-PCR efficiency, sensitivity and specificity in with a simplified protocol. These kits offer reliable and unbiased amplification of all RNA targets regardless of abundance. The proprietary supermix maximises activities of both reverse transcriptase and AccuStart™ <i>Taq</i> DNA polymerase without compromising specificity of amplification. qScript™ One-Step SYBR® Green qRT-PCR kits are available and optimised for all real-time PCR instrument platforms including those requiring normalisation with ROX reference dye.

Artikel nummer : (733-2265)

Leverantör: Quantabio

Beskrivning: PerfeCTa® PreAmp SuperMix is a 5X concentrated, ready to use reaction cocktail for unbiased, selected enrichment of target sequences from limited amounts of starting material for downstream gene expression profiling or targeted resequencing. It contains all components, except primers and templates. The 5X concentrated SuperMix allows addition of higher template volumes when working with low concentration samples, and/or reduced reaction volumes. Inclusion of an inert light blue tracer dye helps visualise small reaction volumes and ensure accurate pipetting.

UOM: 1 * 40 Assays

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Leverantör: Quantabio

Beskrivning: PerfeCTa® MultiPlex qPCR ToughMix® is a 5X concentrated, ready to use reaction cocktail for Real-Time quantitative PCR (qPCR) that overcomes many known inhibitors of PCR often present in crude samples extracted from environmental specimens, plant tissues, or animal tissues. It contains all components, except primers, probes and templates. The 5X concentrated ToughMix® allows addition of higher amounts of template and improved detection sensitivity with low concentration samples. PerfeCta® MultiPlex qPCR ToughMix® has been optimised to deliver maximum PCR efficiency, sensitivity, and specificity in reduced reaction volumes with fast cycle or conventional PCR cycling protocols.

Säkerhetsdatablad Certifikat

Leverantör: Quantabio

Beskrivning: PerfeCTa® qPCR SuperMixes i 2X koncentration är reaktionsblandningar som är färdiga att använda och som innehåller alla komponenter förutom primrar, prob(er) och templat för qPCR.

Leverantör: Quantabio

Beskrivning: The sparQ DNA Frag and Library Prep kit optimises the integration of enzymatic fragmentation into a two-step protocol for the streamlined construction of libraries for sequencing on Illumina® NGS platforms. A single-tube enzyme mix facilitates the combination of fragmentation and DNA polishing minimising DNA over fragmentation while greatly simplifying library.

Leverantör: Quantabio

Beskrivning: Extracta™ DBS is a ready to use DNA extraction reagent for rapid and efficient recovery of PCR-ready DNA from dried blood spots (DBS) on Guthrie cards or Whatman® 903 filter/TFN or 226 from Ahlstrom Munksjö paper. This patented single-solution process produces DNA eluates that are substantially free of PCR inhibitors and compatible with a variety of end-point PCR, real-time PCR and Next Generation Sequencing (NGS) or Sanger Sequencing reagents. Application of Extracta DBS with PerfeCTa® qPCR ToughMix® or PerfeCTa MultiPlex qPCR ToughMix® enables accurate and reproducible quantification of DNA sequences in blood using TaqMan® hydrolysis probe real-time qPCR.

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Leverantör: Quantabio

Beskrivning: AccuStart™ II <i>Taq</i> DNA Polymerase is a high purity, recombinant <i>Taq</i> DNA polymerase preparation with high avidity monoclonal antibodies that bind the polymerase and keep it inactive prior to the initial PCR denaturation step. Upon heat activation (1 minute at 94 °C), the antibodies denature irreversibly, releasing fully active, unmodified <i>Taq</i> DNA polymerase. This enables specific and efficient primer extension with the convenience of room temperature reaction assembly. Non-specific extension of primers at low temperatures is a common cause of artifacts and poor sensitivity in PCR. The AccuStart™ II automatic hot-start enables specific and efficient primer extension in the PCR process with the added convenience of room temperature reaction assembly. The included 10X PCR Buffer II is a new optimised buffer that provides higher product yield, improved specificity, and enhanced multiplexing capability.

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Leverantör: Quantabio

Beskrivning: Superior speed and inhibitor tolerance.

Certifikat

Leverantör: Quantabio

Beskrivning: FastMixes innehåller buffertar och stabiliseringsmedel som optimerats för högsta PCR-effektivitet, reproducerbarhet och känslighet tillsammans med TaqMan® och TaqMan® MGB-prober. En nyckelkomponent i PerfeCTa® qPCR FastMixes är AccuFast™ Taq DNA-polymeras som möjliggör omedelbar aktivering och snabb polymerisationskinetik. Vid värmeinaktivering (20 sekunder vid 95 °C) denatureras antikropparna irreversibelt och frigör fullt aktivt, omodifierat Taq DNA-polymeras. Detta underlättar specifik och effektiv primerextension i rumstemperatur. FastMixes ger maximal PCR-effektivitet, -känslighet och -specificitet och robust fluorescerande signal med antingen snabbt eller konventionellt cyklingsprotokoll.

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