Du sökte efter: Western Blotting Reagents

Leverantör: Merck

Beskrivning: The Visualizer Western Blot Detection kit is designed for the sensitive detection of proteins in chemiluminescent Western blots.

Artikel nummer : (MILF2502)

Leverantör: Merck

Beskrivning: Western blotting is a commonly used technique for studying protein function and localisation. Typically, protein samples are electro-phoresed on SDS-page and transferred to a membrane such as nitrocellulose or nylon, where they are probed with specific antibodies. Unlike nucleic acid based technologies, which allow reuse of southern and northern blots, it has been difficult to reuse western blots.

UOM: 1 * 25 mL

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Leverantör: G-Biosciences

Beskrivning: These are ready to screen, normal tissue, heart tissue region blots from human and pig. The heart tissue specific regions are prepared by carefully dissecting out anatomically and functionally distinct regions of adult pig and adult human hearts.

Leverantör: G-Biosciences

Beskrivning: G-Biosciences offers ready-to-screen, normal tissue, brain tissue region blots. The brain tissue specific regions are prepared by carefully dissecting out anatomically and functionally distinct regions of adult mouse, rat, human, and primate brains.

Artikel nummer : (MILF2060)

Leverantör: Merck

Beskrivning: Western blotting is a commonly used technique for studying protein function and localisation. Typically, protein samples are electro-phoresed on SDS page and transferred to a membrane such as nitrocellulose or nylon, where they are probed with specific antibodies. Unlike nucleic acid based technologies, which allow reuse of southern and northern blots, it has been difficult to reuse western blots.

UOM: 1 * 1 Items

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Leverantör: G-Biosciences

Beskrivning: SWIFT™ transfer pads are treated with a proprietary electrolyte buffer to enhance Western blot transfer efficiency.

Leverantör: G-Biosciences

Beskrivning: Ready-to-screen, human, single tissue blots, from both normal and tumour tissues. G-Biosciences pre-made blots are prepared with the appropriate GenLysate™ and 50 μg protein is loaded onto a 4 to 20% denaturing polyacrylamide gel, along with a prestained molecular weight marker. After the proteins are resolved, they are transferred to a PVDF membrane, using our Western transfer buffer.

Leverantör: Biotium

Beskrivning: NucView® caspase-3 substrates are fluorescent probes that allow for the real-time detection of apoptosis by monitoring caspase-3/7 activity in intact cells using confocal microscopy, flow cytometry or microplate readers.

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Artikel nummer : (BB01)

Leverantör: G-Biosciences

Beskrivning: G-Biosciences' Botanical Garden Blot is prepared using total protein extracted from a variety of 5 to 10 day old, whole plant tissues.

UOM: 1 * 1 Items

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Leverantör: Rockland Immunochemicals

Beskrivning: NPP ELISA alcaline phosphatase substrate is a chromogenic substrate used in immunosorbent assays.

Leverantör: Thermo Fisher Scientific

Beskrivning: SuperSignal™ West Femto-substrat med maximal känslighet för detektion av låga femtogrammängder av protein utfällt på nitrocellulosa- eller PVDF-membran och undersökt med lämpliga primära och sekundära antikroppar.

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Leverantör: Thermo Fisher Scientific

Beskrivning: Vissa antikroppar är svåra att avlägsna från Western-blots och kräver längre inkubationstider eller inkubationstemperaturer som är högre än 22 °C. Restore PLUS™-strippningsbuffert för Western-blotting har utvecklats för att minska inkubationstiderna för inkubation vid rumstemperatur. Högaffinitetsantikroppar kan snabbt och effektivt avlägsnas från Western-blots utan att man tar bort överförda proteiner, vilket innebär att man kan göra flera probningar av målet.

Artikel nummer : (PIER34035)

Leverantör: Thermo Fisher Scientific

Beskrivning: NBT (nitro-blue tetrazolium chloride) yields a blue-purple, water-insoluble product upon reduction of the tetrazolium salt. The product of reduction is NBT-formazan. This substrate is widely used for immunochemistry applications because the color is linear and stable. NBT is used as the substrate for both glucose oxidase and alkaline phosphatase systems.

UOM: 1 * 1 g

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Leverantör: Thermo Fisher Scientific

Beskrivning: Guardian™ Peroxidase conjugate stabiliser/diluent preserves the functional integrity and activity of horseradish peroxidase conjugated antibodies and other proteins at very dilute concentrations for long term storage.

Leverantör: Thermo Fisher Scientific

Beskrivning: The Fast Western Blot Kit, ECL Substrate contains optimised reagents that shorten the time which is required to perform a typical chemiluminescent Western blot from 4 hours down to approximately 55 minutes.

Leverantör: Thermo Fisher Scientific

Beskrivning: The SuperSignal™ West Dura Extended Duration Substrate is a sensitive, luminol-based enhanced chemiluminescent substrate for detecting horseradish peroxidase (HRP) on immunoblots. SuperSignal West Dura Substrate enables mid-femtogram detection of antigen by oxidising luminol in the presence of HRP and peroxide. This reaction produces a prolonged chemiluminescence which can be visualised on X-ray film or an imaging system. Optimal signal intensity and duration can be attained with appropriate primary and secondary antibody dilutions.

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