PopCulture® reagent
Leverantör: Merck Millipore (Novagen)
PopCulture® Reagent is a detergent-based concentrate that can be added directly to cultures of E. coli to effectively extract proteins without the need for cell harvest. Recombinant proteins can be directly screened in the crude extract, or purified by adding an affinity matrix, washing the matrix-target protein complex to remove spent culture medium and cellular contaminants, and eluting the purified protein from the matrix. The PopCulture protein purification procedure is ideally suited to high-throughput robotic processing of samples for proteomics research or any application that would benefit from the increased speed and convenience.
- Culturing, extraction and purification can all be performed in the original culture tube or multiwell plate, without the need for columns or centrifugation
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