Du sökte efter: Merck Millipore (Novagen)

Artikel nummer : (71281-3)

Leverantör: Merck Millipore (Novagen)

Beskrivning: ProteoJuice™ protein transfection reagent is an effective reagent for the transfection of intact functional protein and peptides into mammalian cells with minimal toxicity and broad cell specificity.

UOM: 1 * 1 Items

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Leverantör: Merck Millipore (Novagen)

Beskrivning: The pSTBlue-1 AccepTor™ vector kits are designed to simplify the PCR cloning process by using non-proofreading thermostable DNA polymerases (eg. KOD XL polymerase and native and recombinant <i>Taq</i> polymerases), which leave single 3'-dA overhangs on the reaction products. The AccepTor™ vectors enable direct ligation by virtue of single 3'-dU overhangs that anneal efficiently with 3'-dA extensions on PCR products. The dU residues are converted to dT residues<i> in vivo</i> following transformation.

Artikel nummer : (70856-3)

Leverantör: Merck Millipore (Novagen)

Beskrivning: Convenient solution for selective degradation of RNA.

UOM: 1 * 1 mL

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Leverantör: Merck Millipore (Novagen)

Beskrivning: D-Tube96™ Dialysers allow convenient, high throughput dialysis of 96 samples simultaneously. The device features the advantages of the D-Tube™ Dialyzer Mini in a 96-tube format. D-Tube Dialysers are easy to handle tubes with dual membranes providing a large surface area for fast, efficient dialysis. The membrane is ultra clean, EDTA-treated, regenerated cellulose that is sulfur and heavy metal-free.

Artikel nummer : (70527-3)

Leverantör: Merck Millipore (Novagen)

Beskrivning: The lac operon inducer IPTG (isopropyl-β-D- 1-thiogalactopyranoside) is recommended for blue/white screening by lacZ α- complementation with appropriate vectors and host strains, such as pSTBlue-1/NovaBlue, and for inducing protein expression with the pET System and other lac promoter-derived expression systems.

UOM: 1 * 15 mL

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Leverantör: Merck Millipore (Novagen)

Beskrivning: BugBuster® Plus Lysonase™ includes BugBuster Protein Extraction Reagent and Lysonase Bioprocessing Reagent, an optimised, ready-to-use blend of rLysozyme™ and Benzonase® Nuclease in a single kit. This powerful combination of two reagents allows maximum recovery of active soluble protein from both Gram-negative and Gram-positive bacteria. The BugBuster® and rLysozyme™ efficiently lyse the bacterial cells while the Benzonase® degrades nucleic acids to facilitate downstream processing. Requires 5 ml BugBuster and 10 µl Lysonase per 1 g cell paste. The kits provide sufficient reagents for protein extraction from 20 g or 100 g cell paste.

Leverantör: Merck Millipore (Novagen)

Beskrivning: BugBuster® HT Protein Extraction Reagent combines BugBuster® Protein Extraction Reagent and Benzonase® Nuclease in one convenient reagent. BugBuster® HT eliminates common bioprocessing problems resulting from traditional cell lysis procedures. Soluble proteins are gently extracted from <i>E. coli</i> without exposure to heat or oxidative damage and viscosity is eliminated by nucleic acid digestion in a single step. The resulting protein extract can easily be fractionated by conventional purification techniques. BugBuster HT is ideally suited for application in high throughput protein purifications.

Artikel nummer : (69755-3)

Leverantör: Merck Millipore (Novagen)

Beskrivning: The His·Bind buffer kit is a set of pre-tested buffers designed for use with IDA-based His·Bind resin for convenient, rapid one-step purification of proteins by metal chelation chromatography.

UOM: 1 * 1 KIT

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Artikel nummer : (69750-3)

Leverantör: Merck Millipore (Novagen)

Beskrivning: The pET-24a-d(+) vectors carry an N-terminal T7-Tag® sequence plus an optional C-terminal His-Tag® sequence. These vectors differ from pET-21a-d(+) only by their selectable marker (kanamycin vs ampicillin resistance).

UOM: 1 * 10 µG

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Artikel nummer : (69704-4)

Leverantör: Merck Millipore (Novagen)

Beskrivning: S-protein agarose specifically retains STag™ fusion proteins. The capacity of S-protein agarose varies and is based on the size and folding characteristics of a given target protein.

UOM: 1 * 10 mL

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Leverantör: Merck Millipore (Novagen)

Beskrivning: A common method for transformation of plasmids into E.coli is the use of chemically competent cells. Although competent cells can be prepared in the laboratory, greater efficiency, reproducibility, and convenience are achieved using Novagen® prepared competent cells. Every Novagen® competent cell strain is verified for phenotype and purity, and is guaranteed for transformation efficiency.

Artikel nummer : (71007-3)

Leverantör: Merck Millipore (Novagen)

Beskrivning: The NovaTaq PCR Master Mix is a ready to use 2X concentrated mixture of NovaTaq DNA polymerase, ultra-pure deoxynucleotides, and reaction buffer without magnesium chloride. The master mix simplifies the assembly of PCR reactions and offers advantages of times savings, consistency, and minimal risk of contamination. Simply add the NovaTaq PCR master mix to an equal volume containing the required amount of magnesium chloride, DNA template, and primers, and the reaction is ready for thermal cycling. The final diluted reaction contains 2,5 units of NovaTaq DNA polymerase per 100 µl. Sufficient components are included for 200 standard 50 µl (or 100×100 µl) amplification reactions.

UOM: 1 * 250 EEZ

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Artikel nummer : (69752-3)

Leverantör: Merck Millipore (Novagen)

Beskrivning: The pET-24a-d(+) vectors carry an N-terminal T7·Tag® sequence plus an optional C-terminal His·Tag® sequence.

UOM: 1 * 10 µG

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Artikel nummer : (71115-4)

Leverantör: Merck Millipore (Novagen)

Beskrivning: RiboJuice™ siRNA Transfection Reagent delivers siRNA into a wide range of mammalian cell lines for targeted gene suppression. It is compatible with GeneJuice® Transfection Reagent for cotransfection of siRNA and plasmid DNA for normalising transfection efficiency with a reporter gene.

UOM: 1 * 1 mL

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Leverantör: Merck Millipore (Novagen)

Beskrivning: The Overnight Express™ Autoinduction Systems enable regulated protein expression in <i>E. coli</i>, without monitoring the culture or adding inducer during cell growth.

Leverantör: Merck Millipore (Novagen)

Beskrivning: rLysozyme™ solution contains a highly purified and stabilised recombinant lysozyme that can be used for lysis of gram negative bacteria, such as <i>E. coli</i>.