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Du sökte efter: Reagens för proteinextraktion

Efficient cell lysis and maximum protein extraction yields are vital to high-quality recombinant protein purification. The protease inhibitor cocktails are for lysing mammalian cells, buffers that solubilize plasma and intracellular membranes, break weak intermolecular bonds, and solubilize most proteins. Making procedures easier to perform, these protein extraction reagents can also be used to isolate proteins from both the cytoplasmic and nuclear fractions of cultured cells.

Efficient cell lysis and maximum protein extraction yields are vital to high-quality recombinant protein purification. The protease inhibitor cocktails are for lysing mammalian cells, buffers that solubilize plasma and intracellular membranes, break weak intermolecular bonds, and solubilize most proteins. Making procedures easier to perform, these protein extraction reagents can also be used to isolate proteins from both the cytoplasmic and nuclear fractions of cultured cells.


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Product Image-PIER20230

Immobilised trypsin, TPCK treated, Pierce™

Artikel nummer : (PIER20230)
Leverantör: Thermo Fisher Scientific
Beskrivning: Pierce™ immobilised TPCK trypsin is a serine endoprotease applicable to amino acid analysis and protein sequencing, mapping and structural studies. The immobilised form allows sample separation after treatment.
UOM: 1 * 2 mL
Product Image-PIER28340

Detergent sampler Kit, Surfact-Amps™

Artikel nummer : (PIER28340)
Leverantör: Thermo Fisher Scientific
Beskrivning: Surfact-Amps™ Detergent sampler is a collection of various Surfact-Amps™ detergent solutions and selected other useful detergents for protein research methods.
UOM: 1 * 1 KIT

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Product Image-PIER90402

Mass tagging, mass tag labelling reagents, AminoxyTMT™, Pierce™

Leverantör: Thermo Fisher Scientific
Beskrivning: The carbonyl-reactive Thermo Scientific™ aminoxyTMT™ (Tandem Mass Tag™) Label Reagents enable multiplexed characterisation and quantitation of carbonyl-containing biomolecules (carbohydrates, steroids, oxidised proteins) by mass spectrometry (MS).

Product Image-786-792

Immobilised Trypsin

Artikel nummer : (786-792)
Leverantör: G-Biosciences
Beskrivning: Trypsin is a serine endopeptidase that specifically cleaves peptide bonds on the carboxy side of s-aminoethyl cysteine, arginine and lysine residues and typically there is little or no cleavage at arginyl-proline and lysyl-proline bonds. The distribution of these residues in proteins allows trypsin digestion to produce peptides that are readily identified by mass spectrometry.
UOM: 1 * 1 Items
Product Image-PIER85113

Detergent solutions, Surfact-Amps™

Leverantör: Thermo Fisher Scientific
Beskrivning: Surfact-Amps™ detergents are ready-to-use, diluted solutions of purified detergent that can be used in routine and high-demand protein research methods and molecular biology techniques. These detergents are useful as a component of cell lysis buffers or other solutions intended to extract and solubilise proteins, improve chromatography, prepare yeast spheroplasts, and reduce background in ELISA or Western blot methods.

Image Unavailable-324713-50

Endoproteinase Glu-C, excision grade, Staphylococcus aureus

Artikel nummer : (324713-50)
Leverantör: Merck Millipore (Calbiochem‎)
Beskrivning: Native endoproteinase Glu-C from <i>Staphylococcus aureus</i>. Serine protease that specifically hydrolyses peptide bonds at the carboxylic side of glutamic acid and aspartic acid residues. Inhibited by DFP and α2-macroglobulin. Suggested working concentration: 1:20 to 1:100 (protease:protein by weight) for sequence analysis.
UOM: 1 * 50 µG
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PopCulture® reagent

Leverantör: Merck Millipore (Novagen)
Beskrivning: PopCulture® Reagent is a detergent-based concentrate that can be added directly to cultures of <i>E. coli</i> to effectively extract proteins without the need for cell harvest. Recombinant proteins can be directly screened in the crude extract, or purified by adding an affinity matrix, washing the matrix-target protein complex to remove spent culture medium and cellular contaminants, and eluting the purified protein from the matrix. The PopCulture protein purification procedure is ideally suited to high-throughput robotic processing of samples for proteomics research or any application that would benefit from the increased speed and convenience.

Product Image-PIER90305

Trypsin protease, MS grade

Leverantör: Thermo Fisher Scientific
Beskrivning: Pierce™ Trypsin Protease, MS Grade, is highly purified porcine trypsin that has been chemically modified for maximum activity and stability in proteomic applications.

Certifikat

Image Unavailable-71456-4

Master mix, BugBuster®

Leverantör: Merck Millipore (Novagen)
Beskrivning: BugBuster® master mix combines BugBuster® protein extraction reagent with Benzonase® Nuclease and rLysozyme™ solution in one convenient reagent. It allows for maximum recovery of active soluble protein from both Gram-negative and Gram-positive bacteria. The master mix provides sufficient reagents for protein extraction from 20 g and 100 g cell paste.

Product Image-71009-4

Protein extraction reagents, CytoBuster™

Leverantör: Merck Millipore (Novagen)
Beskrivning: The CytoBuster™ protein extraction reagent is a proprietary formulation of detergents optimised for efficient extraction of soluble proteins from mammalian and insect cells. The gentle, non-ionic composition of the reagent enables isolation of functionally active endogenous or expressed proteins without a need for secondary treatment such as sonication or freeze/thaw.

Image Unavailable-324708-2

Endoproteinase Asp-N, excision grade, Pseudomonas fragi

Artikel nummer : (324708-2)
Leverantör: Merck Millipore (Calbiochem‎)
Beskrivning: Native endoproteinase Asp-N from <i>Pseudomonas fragi.</i> Designed for protein sequencing or sequence verification, analysis of protein structural domains, and cleavage of fusion proteins. Metalloprotease that specifically hydrolyses proteins at the N-terminal side of aspartic acid and cysteic acid residues. Inhibited by aprotinin, DFP, leupeptin, and TLCK. Suggested working concentration: 1:20 to 1:100 (protease:protein by weight) for sequence analysis.
UOM: 1 * 2 µG
Product Image-786-680

Washing solution, Protein-OUT™

Artikel nummer : (786-680)
Leverantör: G-Biosciences
Beskrivning: Protein-OUT™ is a washing solution that cleans plastic and glassware used in the proteolytic digestion and peptide isolation for mass spectrometry analysis. The rinsing of plastic and glassware with Protein-OUT™ will remove interfering chemicals and peptides that can leach from the tube walls and interfere or suppress the mass spectrometry signal. In addition, rinsing with Protein-OUT™ removes inadvertent contamination with dust and keratin.
UOM: 1 * 50 mL
Product Image-REST25849

QuEChERS extraction salts, Q-sep®

Artikel nummer : (REST25849)
Leverantör: Restek
Beskrivning: QuEChERS methods are fast, easy, and cost-effective, and Restek Q-sep® products make QuEChERS procedures even simpler.
UOM: 1 * 50 Items
Image Unavailable-VARIPL6204-6103

Uniform latex particles for immobilisation of biomolecules, PL-Latex

Leverantör: VARIAN
Beskrivning: Uniform latex particles are used in a wide range of applications that exploit their physico-chemical properties in sophisticated immunoassay formats, both in medicine and veterinary science research.

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Lager för denna produkt är begränsat, men kan vara tillgänglig i ett lager nära dig. Se till att du är inloggad på hemsidan så att tillgängligt lager kan visas. Om call fortfarande visas och du behöver hjälp, ring oss på 08-621 34 20.
VWR International AB säljer bara etanol till skattebefriade kunder. Denna produkt ä reglerad enligt svensk lagstiftning. Om ytterligare information krävs av dig kommer du att kontaktas via e-post.
VWR International AB säljer bara etanol till skattebefriade kunder.
Denna produkt ä reglerad enligt svensk lagstiftning. Om ytterligare information krävs av dig kommer du att kontaktas via email.
Produkten har blockerats av din organisation. Vänligen kontakta din inköpsavdelning för mer information.
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Denna produkt är inte längre tillgänglig. Alternativ kan vara tillgängliga genom att söka med VWR art.nr ovan. Om du behöver ytterligare hjälp, ring VWR Kundservice på 08-621 34 20.
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