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Du sökte efter: Reagens för proteinextraktion

Efficient cell lysis and maximum protein extraction yields are vital to high-quality recombinant protein purification. The protease inhibitor cocktails are for lysing mammalian cells, buffers that solubilize plasma and intracellular membranes, break weak intermolecular bonds, and solubilize most proteins. Making procedures easier to perform, these protein extraction reagents can also be used to isolate proteins from both the cytoplasmic and nuclear fractions of cultured cells.

Efficient cell lysis and maximum protein extraction yields are vital to high-quality recombinant protein purification. The protease inhibitor cocktails are for lysing mammalian cells, buffers that solubilize plasma and intracellular membranes, break weak intermolecular bonds, and solubilize most proteins. Making procedures easier to perform, these protein extraction reagents can also be used to isolate proteins from both the cytoplasmic and nuclear fractions of cultured cells.


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Product Image-786-159

Detergent removal system, DetergentOUT™ GBS10

Leverantör: G-Biosciences
Beskrivning: Detergents are essential for protein solubility during protein extraction and sample preparation, especially when working with hydrophobic proteins. The presence of high concentrations of detergents in protein samples can impair ELISA, IEF, protease digestion of proteins and suppress peptide ionisation when analysed by mass spectrometry.

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Image Unavailable-ENZOBMLKI1050300

Fluor-de-Lys® Developer I concentrate

Artikel nummer : (ENZOBMLKI1050300)
Leverantör: ENZO LIFE SCIENCES AG
Beskrivning: FLUOR DE LYS® Developer is an essential reagent for signal generation in HDAC or sirtuin assays done with the FLUOR DE LYS® substrate. An appropriate FLUOR DE LYS® family substrate, comprising an acetylated lysine side chain, is first incubated with a sample containing HDAC activity (nuclear or cellular extract, purified enzyme, bead-bound immunocomplex, etc.). Deacetylation of the substrate sensitizes it so that, in a second step, treatment with the FLUOR DE LYS® Developer produces a fluorophore. Typically, an ‘inhibitor stop’ will be included in the 1x Developer dilution (e.g. Trichostatin A) for class I and II HDACs, nicotinamide for sirtuins).
UOM: 1 * 300 µl

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Protein quantitation reagent, TMTzero™ label reagent

Artikel nummer : (PIER90067)
Leverantör: Thermo Fisher Scientific
Beskrivning: Pierce™ TMTzero™ Label Reagent serves as a "light" tag for MS-level peptide quantitation similar to duplex isotopic metabolic labeling, such as SILAC, or isotopic dimethylation labeling.
UOM: 1 * 1 KIT
Product Image-PIER88701

Universal nuclease for cell lysis, Pierce™

Leverantör: Thermo Fisher Scientific
Beskrivning: The Pierce™ Universal Nuclease for Cell Lysis is ideal for a wide variety of applications where complete digestion of nucleic acids is needed when preparing cell lysates.

Image Unavailable-ENZOBMLKI1761250

Fluor-de-Lys® Developer II concentrate

Artikel nummer : (ENZOBMLKI1761250)
Leverantör: ENZO LIFE SCIENCES AG
Beskrivning: FLUOR DE LYS® Developer II is an essential reagent for signal generation in assays done with the FLUOR DE LYS®-HDAC8, FLUOR DE LYS®-SIRT1 and FLUOR DE LYS®-SIRT2 substrates. Deacetylation of the FLUOR DE LYS® substrate sensitises it so that, in a second step, treatment with the FLUOR DE LYS® Developer II produces a fluorophore. Typically, an ‘inhibitor stop’ will be included in the 1X Developer dilution (e.g. nicotinamide for sirtuins).
UOM: 1 * 1,25 mL

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Antigen retrieval reagent, IHC

Leverantör: ENZO LIFE SCIENCES AG
Beskrivning: Antigen retrieval reagent is used to recover antigens masked by fixation in cross linking fixatives such as formalin.

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Product Image-786-244

SilverOUT™, G-Biosciences

Artikel nummer : (786-244)
Leverantör: G-Biosciences
Beskrivning: SilverOUT removes interfering silver ions from gel pieces.
UOM: 1 * 100 Tests
Product Image-786-793

Immobilised Ficin

Artikel nummer : (786-793)
Leverantör: G-Biosciences
Beskrivning: Ficin (or Ficain) is a cysteine protease enzyme (EC 3.4.22.3) isolated from fig latex is that has the endopeptidase activity to cleave Immunoglobulin G (IgG) molecules in the hinge region.
UOM: 1 * 5 mL
Product Image-786-598

Biotin resin

Artikel nummer : (786-598)
Leverantör: G-Biosciences
Beskrivning: Immobilised biotin resin is designed for the high affinity chromatography purifications of avidin, streptavidin and neutravidin protein. The resin consists of biotin coupled to 6% cross-linked agarose.
UOM: 1 * 5 mL
Product Image-DGA01

Optimizer blueBALLS™

Artikel nummer : (DGA01)
Leverantör: G-Biosciences
Beskrivning: The 'critical micelle concentration' (CMC) of a detergent varies with temperature, pH, ionic strength, detergent concentration, purity and presence of organic agents in the detergent. Using a large excess of detergent may pose problems during purification procedures or other downstream applications.
UOM: 1 * 500 Items
Product Image-786-681

InGel™ Blue for protein digestion

Artikel nummer : (786-681)
Leverantör: G-Biosciences
Beskrivning: InGel™ Blue provides a complete set of reagents for the in-gel tryptic digestion and extraction of peptides for mass spectrometry (MALDI and LC MS/MS). The kit is specifically designed for use with Coomassie® or fluorescent stained protein spots/bands.
UOM: 1 * 1 KIT
Product Image-PIER90054

Endoproteinase Glu-C protease, MS grade, Pierce™

Artikel nummer : (PIER90054)
Leverantör: Thermo Fisher Scientific
Beskrivning: Glu-C protease is a highly specific endoproteinase use to improve sequence coverage in mass spectrometry protein identification applications.
UOM: 1 * 50 µG

Säkerhetsdatablad


Product Image-PIER90056

Endoproteinase chymotrypsin protease (TLCK treated), MS grade, Pierce™

Artikel nummer : (PIER90056)
Leverantör: Thermo Fisher Scientific
Beskrivning: Chymotrypsin protease is a purified native endoproteinase validated for maximum activity and selectivity in proteomic applications.
UOM: 1 * 100 µG
Product Image-786-599

Immobilised Iminobiotin Resin

Artikel nummer : (786-599)
Leverantör: G-Biosciences
Beskrivning: Immobilised Iminobiotin Resin consists of iminobiotin, a cyclic guanido analog of biotin, covalently coupled to 6% crosslinked agarose. The resin allows for the purification of avidin, streptavidin and Neutravidin and their subsequent gentle elution using non denaturing elution buffers.
UOM: 1 * 5 mL
Product Image-PIER90053

Endoproteinase Asp-N protease, MS grade, Pierce™

Artikel nummer : (PIER90053)
Leverantör: Thermo Fisher Scientific
Beskrivning: Asp-N protease is a highly specific endoproteinase used to improve sequence coverage in mass spectrometry protein identification applications.
UOM: 1 * 2 µG

Säkerhetsdatablad


Product Image-786-077

Protein reducing agents

Leverantör: G-Biosciences
Beskrivning: Protein-S-S-Reductant™ uses TCEP (Tris [2-carboxyethyl] phosphine), a popular alternative to β-mercaptoethanol and DTT (dithiothreitol). TCEP improves stability, increases effectiveness, and reduces proteins over a wider range of pH, including lower acidic pHs. Protein-S-S-Reductant™ completely reduces stable disulfide bonds in less than 5 minutes at room temperature and is compatible with the protein alkylation reactions. This ready to use solution is at a neutral pH and stabilised for long-term storage. Simply supplement Protein-S-S-Reductant™ in place of DTT or β-mercaptoethanol and boil the sample.

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Lager för denna produkt är begränsat, men kan vara tillgänglig i ett lager nära dig. Se till att du är inloggad på hemsidan så att tillgängligt lager kan visas. Om call fortfarande visas och du behöver hjälp, ring oss på 08-621 34 20.
Lager för denna produkt är begränsat, men kan vara tillgänglig i ett lager nära dig. Se till att du är inloggad på hemsidan så att tillgängligt lager kan visas. Om call fortfarande visas och du behöver hjälp, ring oss på 08-621 34 20.
VWR International AB säljer bara etanol till skattebefriade kunder. Denna produkt ä reglerad enligt svensk lagstiftning. Om ytterligare information krävs av dig kommer du att kontaktas via e-post.
VWR International AB säljer bara etanol till skattebefriade kunder.
Denna produkt ä reglerad enligt svensk lagstiftning. Om ytterligare information krävs av dig kommer du att kontaktas via email.
Produkten har blockerats av din organisation. Vänligen kontakta din inköpsavdelning för mer information.
Lösenordet och säkerhetsfrågan associerade med ditt Kundnummer har gått ut. Vänligen ändra ditt lösenord och din säkerhetsfråga.
Denna produkt är inte längre tillgänglig. Alternativ kan vara tillgängliga genom att söka med VWR art.nr ovan. Om du behöver ytterligare hjälp, ring VWR Kundservice på 08-621 34 20.
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