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Du sökte efter: Western Blotting Reagents

Nucleic acids or proteins in a complex sample can be visualized using electrophoresis and blotting procedures and the compatible reagents. Improving the overall efficiency of electrophoresis systems, these reagents accommodate a variety of blotting techniques and with antibodies provided against tens of thousands of different proteins. These methods are used in the fields of molecular biology, immunogenetics, and other molecular biology disciplines.

Nucleic acids or proteins in a complex sample can be visualized using electrophoresis and blotting procedures and the compatible reagents. Improving the overall efficiency of electrophoresis systems, these reagents accommodate a variety of blotting techniques and with antibodies provided against tens of thousands of different proteins. These methods are used in the fields of molecular biology, immunogenetics, and other molecular biology disciplines.


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Product Image-PIER34065

DAB substrate kit, metal enhanced, Pierce™

Artikel nummer : (PIER34065)
Leverantör: Thermo Fisher Scientific
Beskrivning: The Pierce™ Metal enhanced DAB substrate kit uses cobalt and nickel chloride in a special formulation of diaminobenzidine peroxidase substrate that yields intense colour for immunohistochemical staining.
UOM: 1 * 250 mL
Product Image-PIER15168

West HisProbe kit, SuperSignal™

Artikel nummer : (PIER15168)
Leverantör: Thermo Fisher Scientific
Beskrivning: SuperSignal™ West HisProbe Kit contains HisProbe-HRP conjugate and reagents required for detection of polyhistidine-tagged protein by Western blotting. HisProbe-HRP Conjugate is a nickel (Ni²⁺)-activated derivative of horseradish peroxidase (HRP) that enables direct, IMAC-based detection of His-tagged proteins.
UOM: 1 * 1 KIT

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Product Image-786-123T

Sample clean up, PAGE-Perfect™

Leverantör: G-Biosciences
Beskrivning: Many lysis buffers and reagents used in sample preparation are incompatible with routinely used electrophoretic analysis. The presence of contaminants, or interfering agents, such as salts, acids, bases and detergents, result in band distortion and poor protein resolution. As a result, SDS-PAGE gels are hard to analyse and lack reproducibility. PAGE-Perfect™ is a simple, two-step method for concentrating, cleaning and preparing protein solutions for running publication quality gels.

Image Unavailable-69078-3

Western blotting kit, Perfect Protein™, Novagen®

Artikel nummer : (69078-3)
Leverantör: Merck Millipore (Novagen)
Beskrivning: The Perfect Protein™ HRP Western Blot Kit includes an HRP or AP conjugate in addition to Perfect Protein™ Western markers.
UOM: 1 * 1 Items
Product Image-ROCK18-8815-33

Western blot kits, TrueBlot®

Leverantör: Rockland Immunochemicals
Beskrivning: TrueBlot® Western blot kits contain the critical supporting reagents, buffers, and substrates for immunoprecipitation and Western blotting of samples using TrueBlot® second step immunoblotting reagents in conjunction with the user's own primary IP antibody and primary (mouse, goat or rabbit IgG) Western blotting antibody.

Product Image-ROCKFEMTOMAX-020

Chemiluminescent super sensitive HRP substrate, FemtoMax™

Leverantör: Rockland Immunochemicals
Beskrivning: FemtoMax™ is an extremely sensitive, non radioactive, and enhanced luminol-based chemiluminescent substrate for the detection of horseradish peroxidase (HRP). It allows for the detection of femtogram (10 to 15) amounts of antigen using photographic film or other imaging methods including highly sensitive CCD cameras.

Image Unavailable-J60561.AP

SSC (20X), RNAse free

Leverantör: Thermo Fisher Scientific
Beskrivning: SSC solution is commonly used in nucleic acid hybridization techniques at concentrations ranging from 0,1X to 20X depending on the application. It is used to facilitate transfer of nucleic acids to membranes in Southern and Northern blotting, dot blotting, and colony and plaque lifts.

Säkerhetsdatablad Certifikat

Product Image-PIER35065

Fast western blotting kit, SuperSignal™ west Pico, Pierce™

Leverantör: Thermo Fisher Scientific
Beskrivning: The Fast Western Blot Kit, SuperSignal™ West Pico Substrate contains optimised reagents and protocol for picogram-level detection and and Western blot development less than 1 hour after gel transfer.

Image Unavailable-WB59-100

Western-Re-Probe™ reagent, Calbiochem®

Artikel nummer : (WB59-100)
Leverantör: Merck Millipore (Oncogene)
Beskrivning: This Western-Re-Probe™ reagent is validated for use in immunoblotting.
UOM: 1 * 100 mL
Product Image-786-124

Sample clean up, Perfect-FOCUS™

Leverantör: G-Biosciences
Beskrivning: Perfect-FOCUS™ is based on quantitative precipitation and concentration of protein solutions using UPPA™ (Universal Protein Precipitation Agent) and has been specifically developed for preparing lower conductivity protein samples for iso-electric focusing gels. Perfect-FOCUS™ is designed to clean and remove interfering agents from protein samples and concentrate protein solutions that give poor protein spot resolution during 2D electrophoresis.

Product Image-PIER35081

SuperSignal™, Pierce™ Fast Western Blotting Kit, West Femto

Leverantör: Thermo Fisher Scientific
Beskrivning: The Pierce™ Fast Western Blot Kit, SuperSignal™ West Femto Substrate contains optimised reagents that shorten the time to perform a typical Western blot from 4 hours to ~60 minutes.

Image Unavailable-J63209.AK

Saponin permeating solution, 0,5% w/v solution in PBS (10X)

Artikel nummer : (J63209.AK)
Leverantör: Thermo Fisher Scientific
Beskrivning: Saponin permeating solution, is used as a cell permeabilising agent.
UOM: 1 * 250 mL

Säkerhetsdatablad Certifikat


Image Unavailable-J61462.AK

Luminol and oxidising solutions

Leverantör: Thermo Fisher Scientific
Beskrivning: For chemiluminecent HRP-based detection of western blotting systems. It is soluble in most polar organic solvents but is insoluble in water.

Säkerhetsdatablad Certifikat

Product Image-PIER35071

Fast western blot kit, SuperSignal™ West Dura, Pierce™

Leverantör: Thermo Fisher Scientific
Beskrivning: The Fast Western Blot Kits, SuperSignal™ West Dura provide reagents and protocol for reliable and rapid, 1 hour chemiluminescent Western blotting at femtogram levels of sensitivity. These complete Fast Western Blot Kits requires minimal hands on time and yield results comparable to classical Western blotting with SuperSignal™ West Dura Chemiluminescent Substrate. This substrate produces a signal that is detectable using photographic or other imaging methods. The developed blots can be repeatedly exposed and stripped of the primary antibody and reprobed. The complete kit contains all the reagents necessary to block and probe a Western blot with mouse or rabbit primary antibody.

Product Image-ROCKKCA001

Chemiluminescent western blot kits, FemtoMax™

Leverantör: Rockland Immunochemicals
Beskrivning: These Chemiluminescent Western Blot Kits allow for the detection of primary polyclonal or monoclonal antibodies provided by the user. After protein separation and transfer, the membrane is probed with primary antibody. Detection of the membrane-bound primary antibody-antigen complex is achieved by the addition of a secondary antibody conjugated to the enzyme horseradish peroxidase. The enzyme reacts with a specialised formulation of luminol, an extremely sensitive, non radioactive substrate that emits light and allows visualisation using X-ray film or other imaging methods, including highly sensitive CCD cameras and imaging systems. Because of the extremely high sensitivity of the kit, primary and secondary antibodies can be used at greater dilutions. Primary antibodies currently diluted 1:1000 using an ECL™ substrate is the equivalent of a 1:5000 dilution using the FemtoMax™ substrate.

Product Image-TB110

Kingdom blot

Artikel nummer : (TB110)
Leverantör: G-Biosciences
Beskrivning: Kingdom blot is prepared using total protein extracted from a variety of species, where whole species is not available the whole liver lysate is used. These blots can be used to identify similar proteins in other species, including human, chicken, frog, worm, Drosophila, yeast, Aspergillus, <i>E. coli</i>, Rhizobium and green algae.
UOM: 1 * 1 Items
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Lager för denna produkt är begränsat, men kan vara tillgänglig i ett lager nära dig. Se till att du är inloggad på hemsidan så att tillgängligt lager kan visas. Om call fortfarande visas och du behöver hjälp, ring oss på 08-621 34 20.
Lager för denna produkt är begränsat, men kan vara tillgänglig i ett lager nära dig. Se till att du är inloggad på hemsidan så att tillgängligt lager kan visas. Om call fortfarande visas och du behöver hjälp, ring oss på 08-621 34 20.
VWR International AB säljer bara etanol till skattebefriade kunder. Denna produkt ä reglerad enligt svensk lagstiftning. Om ytterligare information krävs av dig kommer du att kontaktas via e-post.
VWR International AB säljer bara etanol till skattebefriade kunder.
Denna produkt ä reglerad enligt svensk lagstiftning. Om ytterligare information krävs av dig kommer du att kontaktas via email.
Produkten har blockerats av din organisation. Vänligen kontakta din inköpsavdelning för mer information.
Lösenordet och säkerhetsfrågan associerade med ditt Kundnummer har gått ut. Vänligen ändra ditt lösenord och din säkerhetsfråga.
Denna produkt är inte längre tillgänglig. Alternativ kan vara tillgängliga genom att söka med VWR art.nr ovan. Om du behöver ytterligare hjälp, ring VWR Kundservice på 08-621 34 20.
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