Du sökte efter: Protein Extraction Kits

Leverantör: Thermo Fisher Scientific

Beskrivning: B-PER® Bacterial Protein Extraction Reagents are designed to extract soluble protein from bacterial cells. The B-PER® Bacterial Protein Extraction Kit eliminates the need for harsh mechanical procedures, like sonication. The easy-to-use cell lysis solution uses mild non-ionic detergents to disrupt cells and solubilise proteins without denaturation. Enzyme supplements such as DNase I and Lysozyme allow for improved yield of large molecular weight proteins which are difficult to purify. B-PER® is also compatible with downstream protein purification methods such as GST purification and His-tagged immobilised metal affinity chromatography (IMAC).

Leverantör: G-Biosciences

Beskrivning: Antibody Fab and F(ab)₂ Fragmentation of Mouse IgG₁ Kits are designed for the generation and isolation of Fab and F(ab)₂ fragments from mouse IgG₁ molecules. The kit utilises Immobilised Ficin resin. Ficin (or Ficain) (~25000 Da) is a cysteine protease enzyme (EC 3.4.22.3) isolated from fig latex that has the endopeptidase activity to cleave immunoglobulin G molecules in the hinge region. Ficin has an effective range of pH 4 to 9,5 with an optimal pH of 6,5 and cleaves bonds that involve uncharged or aromatic amino acids. Ficin is typically used to cleave mouse IgG₁ as this is difficult to cleave with papain and pepsin. In the presence of 1 to 4 mM or 10 to 20 mM cysteine, ficin generates F(ab)₂ and Fab fragments respectively.

Artikel nummer : (786-257)

Leverantör: G-Biosciences

Beskrivning: Specifically designed for yeast research and supplied with yeast specific reagents. Extracts and solubilises nearly all of the proteins from yeast, including membrane and soluble proteins. Extraction is based on gentle lysis of yeast cells with a yeast lytic enzyme preparation, LongLife™ Zymolyase®, which has improved stability and shelf life. Enzymatic action is followed by extraction of total proteome with the supplied strong chaotropic extraction buffer that solubilises even the most difficult proteins.

UOM: 1 * 1 KIT

Sale

Artikel nummer : (786-249)

Leverantör: G-Biosciences

Beskrivning: FOCUS™ Membrane Proteins for the fractionation of highly enriched membrane proteins.

UOM: 1 * 1 KIT

Sale

Leverantör: G-Biosciences

Beskrivning: Samples that contain a large abundance of albumin, such as plasma and cerebrospinal fluid, tend to mask identification and discovery of other less abundant proteins in two dimensional gel electrophoresis and other studies. AlbuminOUT™ has been specifically developed for substantial removal of albumin from such samples.

Artikel nummer : (786-022)

Leverantör: G-Biosciences

Beskrivning: FOCUS™ Mitochondria kit is specifically designed for the isolation of intact mitochondria from cultured mammalian cells. This kit allows for the fast and efficient fractionation of the cytoplasm of cultured mammalian cells into an enriched fraction of mitochondria. The majority (>90%) of the mitochondria have intact inner and outer membranes and therefore retain their functionality.

UOM: 1 * 1 KIT

Sale

Artikel nummer : (786-258)

Leverantör: G-Biosciences

Beskrivning: Specifically designed for bacterial research and supplied with bacteria specific reagents. Extracts and solubilises nearly all of the proteins from <i>E.coli</i>, including membrane as well as soluble proteins. Extraction is based on the gentle lysis of bacterial cells with LongLife™ Lysozyme enzyme, followed by extraction of total proteome with the supplied strong chaotropic extraction buffer that solubilises even the most difficult proteins.

UOM: 1 * 1 KIT

Sale

Artikel nummer : (786-260)

Leverantör: G-Biosciences

Beskrivning: FOCUS™ SubCell for the fractionation of mitochondrial, nuclear, and cytosolic soluble and membrane proteins.

UOM: 1 * 1 KIT

Sale

Artikel nummer : (786-250)

Leverantör: G-Biosciences

Beskrivning: Lipid rafts are membrane microdomains that are enriched in caveolin, cholesterol, glycolipids, sphingolipids and glycosyl-phosphatidylinositol. Lipid rafts are also known as detergent-insoluble glycolipid-enriched complexes (GEMs) or DIGs. Many signalling proteins, including glycosylphosphatidylinositol (GPI)-anchored proteins, doubly-acylated tyrosine kinases of the Src family, and transmembrane proteins, are located in lipid rafts. Lipid raft localised proteins have been shown to be involved in intracellular membrane trafficking and signalling.

UOM: 1 * 1 KIT

Sale

Artikel nummer : (ENZOENZ450150100)

Leverantör: ENZO LIFE SCIENCES AG

Beskrivning: Rapid, reliable method to isolate nucleic acid-free nuclear proteins in mammalian cells and tissues.

UOM: 1 * 1 KIT

New Product Sale

Artikel nummer : (ENZOENZ450130002)

Leverantör: ENZO LIFE SCIENCES AG

Beskrivning: Rapid, reliable, high throughput method to isolate histone proteins in mammalian cells for histone modification applications.

UOM: 1 * 1 KIT

New Product Sale

Artikel nummer : (786-246)

Leverantör: G-Biosciences

Beskrivning: Extracts and solubilises nearly all of the proteins from mammalian samples, including membrane as well as soluble proteins, by a strong chaotropic extraction buffer to solubilise even the most difficult proteins. Suitable for biological samples from animal tissues and adherent and suspension cells.

UOM: 1 * 1 KIT

Sale

Artikel nummer : (PIER88279)

Leverantör: Thermo Fisher Scientific

Beskrivning: The Pierce™ Mouse Embryonic Fibroblast (MEF) Isolation Kit enables high yield isolation and culture of viable primary fibroblasts from mouse embryos.

UOM: 1 * 1 KIT

Sale

Artikel nummer : (786-018)

Leverantör: G-Biosciences

Beskrivning: FOCUS™ Global Fractionation kit is designed to fractionate complex biological samples into cytosolic and membrane fractions. The resulting membrane fractions are subsequently fractionated into either peripheral and integral membrane proteins or lipid raft associated proteins and detergent soluble membrane proteins.

UOM: 1 * 1 KIT

Sale

Artikel nummer : (786-360)

Leverantör: G-Biosciences

Beskrivning: Extracts and solubilises nearly all of the proteins from insect cell cultures (i.e. Sf9 and Sf21), including membrane as well as soluble proteins, using a strong chaotropic extraction buffer to solubilise even the most difficult proteins.

UOM: 1 * 1 KIT

Sale

Leverantör: Biotium

Beskrivning: AccuOrange™ Protein Quantitation Kit is a highly sensitive fluorescence-based assay for quantitating purified protein samples in 96-well format (excitation/emission = 480/598 nm).