How to make chromatography more sustainable

Liquid chromatography is a widely used analytical technique in various fields such as pharmaeuticals, biotechnology, food and beverage, environmental monitoring and more. Most popular type of liquid chromatography is reversed phase (~>75%), which in general so far it is not a "green" analytical method Up to now it is mainly based on acetonitrile and still uses relatively large columns (the most used column dimensin still is 250x4.6 mm). however, there are a few mobile phase tweaking possibilities how to make chromatography in more sustainable without compromising performance of chromatographical separation.

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