Du sökte efter: Thermo Fisher Scientific

Leverantör: Thermo Fisher Scientific

Beskrivning: Maxima™ H Minus First Strand cDNA Synthesis Kit is a complete system for highly efficient synthesis of first strand cDNA. The kit uses the Maxima™ H Minus Reverse Transcriptase (RT), which is an advanced enzyme derived by<i> in vitro</i> evolution of M-MuLV RT. The enzyme features the highest thermostability among the derivatives of M-MuLV RT and lacks RNase H activity. The Maxima™ H Minus First Strand cDNA Synthesis Kit allows synthesis of long cDNAs up to 20 kb at elevated temperatures (up to 65 °C), superseding other systems' abilities to produce full-length cDNA. Due to increased synthesis rates the reaction can be completed in 30 minutes.

Artikel nummer : (EP0072)

Leverantör: Thermo Fisher Scientific

Beskrivning: <i>Taq</i> DNA Polymerase is a highly thermostable DNA polymerase from the thermophilic bacterium <i>Thermus aquaticus</i>. The enzyme catalyses 5'→3' synthesis of DNA, has no detectable 3'→5' exonuclease (proofreading) activity and possesses low 5'→3' exonuclease activity. In addition, <i>Taq</i> DNA polymerase exhibits deoxynucleotidyl transferase activity, which frequently results in the addition of extra adenines at the 3'-end of PCR products. Native <i>Taq</i> DNA polymerase is preferred for amplification of bacterial DNA sequences homologous to those found in <i>E.coli</i>. Native<i> Taq</i> DNA polymerase stabilised with BSA is often the best choice when amplifying DNA samples of lower purity, e.g. genomic DNA from mouse tail.

UOM: 1 * 1 KIT

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Artikel nummer : (EL0016)

Leverantör: Thermo Fisher Scientific

Beskrivning: This T4 DNA ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA. The enzyme repairs single-strand nicks in duplex DNA, RNA, or DNA/RNA hybrids. It also joins DNA fragments with either cohesive or blunt termini, but has no activity on single-stranded nucleic acids.

UOM: 1 * 1 KIT

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Leverantör: Thermo Fisher Scientific

Beskrivning: RevertAid™ Reverse Transcriptase (RT) is a genetically modified M-MuLV RT. It differs from the M-MuLV RT by its structure and catalytic properties. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity and a RNase H activity specific to RNA in RNA-DNA hybrids, which is significantly lower than that of Avian Myeloblastosis Virus (AMV) reverse transcriptase.

Artikel nummer : (R0242)

Leverantör: Thermo Fisher Scientific

Beskrivning: dNTP mix contains premixed aqueous solutions of dATP, dCTP, dGTP and dTTP, each at a final concentration of 2 mM.

UOM: 1 * 1 KIT

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Artikel nummer : (FD1194)

Leverantör: Thermo Fisher Scientific

Beskrivning: GG site and cuts best at 37 °C in 5 to 15 minutes using universal FastDigest buffer.

UOM: 1 * 1 KIT

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Leverantör: Thermo Fisher Scientific

Beskrivning: Thermo Scientific™ Maxima™ H Minus cDNA Synthesis Master Mix with dsDNase provides a simple workflow that combines genomic DNA elimination and cDNA synthesis in a one-tube procedure. The cDNA reaction components are pre-mixed into a complete master mix that is convenient to use, reduces pipetting steps, and is optimised for cDNA synthesis in two-step quantitative RT-PCR (RT-qPCR) applications. Maxima™ H Minus cDNA Synthesis Master Mix is optimised for reproducible cDNA synthesis at elevated temperatures (50 to 65°C) within 30 minutes.

Leverantör: Thermo Fisher Scientific

Beskrivning: The aLICator™ LIC Cloning and Expression System is designed for fast and efficient ligation independent cloning and tight regulation of gene expression in <i>E. coli</i>. The plate bacterial expression vectors are designed for high levels of target protein expression in concert with minimal basal (uninduced) expression, which permits expression of proteins that are toxic to <i>E. coli</i> cells.

Leverantör: Thermo Fisher Scientific

Beskrivning: DreamTaq™ PCR Master Mix (2X) is a ready to use solution containing DreamTaq™ DNA polymerase, optimised DreamTaq™ buffer, MgCl₂ and dNTPs. This pre-mixed formulation saves time and reduces contamination due to the fewer pipetting steps required for PCR set-up. DreamTaq™ DNA polymerase is an enhanced <i>Taq</i> polymerase optimised for high throughput PCR applications. It ensures robust amplification of PCR products up to 6 kb from genomic DNA and up to 20 kb from viral DNA.

Leverantör: Thermo Fisher Scientific

Beskrivning: DreamTaq Green PCR Master Mix (2X) is a ready to use solution containing DreamTaq™ DNA polymerase, optimised DreamTaq Green buffer, MgCl₂ and dNTPs. The master mix is supplemented with two tracking dyes and a density reagent that allows for direct loading of the PCR product on a gel. The dyes in the master mix do not interfere with PCR performance and are compatible with downstream applications, such as DNA sequencing, ligation and restriction digestion. The master mix retains all features of DreamTaq DNA polymerase. It is capable for robust amplification up to 6 kb from genomic DNA and up to 20 kb from viral DNA.

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Leverantör: Thermo Fisher Scientific

Beskrivning: dNTP mix contains premixed aqueous solutions of dATP, dCTP, dGTP and dTTP, each at a final concentration of 10 mM.

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Leverantör: Thermo Fisher Scientific

Beskrivning: Thermo Scientific™ FastDigest™ enzymes are an advanced line of restriction enzymes for rapid DNA digestion.

Artikel nummer : (FD0454)

Leverantör: Thermo Fisher Scientific

Beskrivning: Thermo Scientific™ FastDigest™ enzymes are an advanced line of restriction enzymes for rapid DNA digestion.

UOM: 1 * 1 KIT

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Leverantör: Thermo Fisher Scientific

Beskrivning: DreamTaq™ DNA Polymerase is an enhanced <i>Taq</i> DNA polymerase optimised for all standard PCR applications. It ensures higher sensitivity, longer PCR products and higher yields compared to conventional <i>Taq</i> DNA polymerase. DreamTaq™ DNA Polymerase uses the same reaction set-up and cycling conditions as conventional <i>Taq</i> DNA polymerase. Extensive optimisation of reaction conditions is not required. The enzyme is inhibited by dUTP but can incorporate modified nucleotides. Applications include routine PCR amplification of DNA fragments up to 6 kb from genomic DNA and up to 20 kb from viral DNA, RT-PCR, and generation of PCR products for TA cloning.

Leverantör: Thermo Fisher Scientific

Beskrivning: HindIII restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions.

Säkerhetsdatablad

Leverantör: Thermo Fisher Scientific

Beskrivning: <i>Taq</i> DNA Polymerase is a highly thermostable DNA polymerase from the thermophilic bacterium <i>Thermus aquaticus</i>. The enzyme catalyses 5'→3' synthesis of DNA, has no detectable 3'→5' exonuclease (proofreading) activity and possesses low 5'→3' exonuclease activity. In addition, <i>Taq</i> DNA polymerase exhibits deoxynucleotidyl transferase activity, which frequently results in the addition of extra adenines at the 3'-end of PCR products. Recombinant <i>Taq</i> DNA Polymerase is ideal for standard PCR of templates 5 kb or shorter.