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Product Image-OZBIET10500

DNA transfection reagent, EcoTransfect™

Beskrivning: EcoTransfect™ transfection reagent is an economical lipofection reagent dedicated to the transfection of popular cell lines. It is the perfect solution for quickly analysing the biological activity of nucleic acids, to perform routine transfection assays at low cost and to accomplish high throughput screening.
Artikel nummer : OZBIET10500
UOM: 1 * 500 µl
Leverantör: OZ BIOSCIENCES
Image Unavailable-31030-50

DNA gel extraction kit

Beskrivning: The DNA Gel Extraction Kit is a silica-gel, spin column based DNA extraction kit designed to purify 40 bp to 40 kb DNA fragments from agarose gels in TAE or TBE buffer.
Artikel nummer : 31030-50
UOM: 1 * 1 KIT
Leverantör: Biotium

Säkerhetsdatablad


Product Image-786-652

RNA purification kit, Tri-Xtract™

Beskrivning: Tri-Xtract™ is designed for the isolation of total RNA that is free from protein and DNA contamination. The isolated RNA is suitable for Northern blots, dot blot hybridisation, <i>in vitro</i> translation, RNase protection assays and poly (A+) selection.
Artikel nummer : 786-652
UOM: 1 * 100 mL
Leverantör: G-Biosciences
Image Unavailable-733-1271

dNTP mix

Beskrivning: The 10 mM dNTP mix is a solution of high purity deoxyribonucleoside triphosphates that has been functionally qualified for real-time quantitative PCR (qPCR). It is suitable for use in conventional end-point PCR, real time qPCR, first-strand cDNA synthesis, as well as other applications that require dNTP as substrate. The use of qPCR certified dNTPs is a simple but important measure to ensure consistent assay performance.
Artikel nummer : 733-1271
UOM: 1 * 1.000 µl
Leverantör: Quantabio
Image Unavailable-ICNA04802511

Etidiumbromid

Beskrivning: Ethidium bromide(EtBr) has been used extensively to reduce mitochondrial DNA copy number in proliferating cells and most commonly used in nucleic acid stain for PAGE or agarose gel electrophoresis. The fluorescence of EtBr increases 21-fold upon binding to double-stranded RNA and 25-fold on binding double-stranded DNA so that destaining the background is not necessary with a low stain concentration (10 μg/mL). Ethidium bromide has been used in a number of fluorimetric assays for nucleic acids. It has been shown to bind to single-stranded DNA (although not as strongly) and triple-stranded DNA.4 Because of its ability to bind to DNA, EtBr is an inhibitor of DNA polymerase. A 10mg/mL easy-to-use solution of ethidium bromide in specially filtered, deionized water which is excellent for nucleic acid electrophoresis and purification applications, eliminates the dust hazard associated with powdered ethidium bromide and saves time spent on weighing and mixing. Used in the fluorometric detection of double stranded nucleic acids in gel electrophoresis. Also acts as an RNA polymerase inhibitor, and in separation of high molecular weight DNA's. It confers a deep red stain to DNA by acting as an intercalating agent between the starch bases. Subsequent fluorescence techniques enable the extraction and characterization of DNA bands. Biochem/physiol Actions: Ethidium bromide intercalates double-stranded DNA and RNA and acts as a frameshift mutagen. It can also be used in conjunction with acridine orange to differentiate between viable, apoptotic and necrotic cells.
Artikel nummer : ICNA04802511
UOM: 1 * 10 mL
Leverantör: MP Biomedicals
Image Unavailable-PRSI33-720

Anti-IFNA2 Mouse Monoclonal Antibody [clone: N27]

Beskrivning: Recognises a protein of 16-27 kDa, identified as human Interferon alpha 2 (IFNA2). Its epitope maps between amino acids 43-53 of the IFNA2. This mAb is specific for IFNA2 and does not cross-react with IFNA1. The epitopes of clones N27 and N39 mAbs are different and represent a good combination of antibodies to set up an ELISA assay for the quantitation of IFNA2 after viral infections. The IFNA family consists of 24 or more genes or pseudo-genes. The alpha interferons are mainly produced by lymphocytes, monocytes, macrophages, and cell lines such as Namalwa and KG1 following induction by viruses, nucleic acids, and glucocorticoid hormones. They are involved in virus resistance on target cells, inhibition of cell proliferation, induction of cytokines and regulation of expression of MHC class I antigens.
Artikel nummer : PRSI33-720
UOM: 1 * 100 µG
Leverantör: ProSci Inc.
Product Image-13-B2013-02

VWR®, Accessories for peqGOLD Kits

Beskrivning: Nucleic Acid Purification Kits and Reagents, Solution I
Artikel nummer : 13-B2013-02
UOM: 1 * 60 mL
Leverantör: VWR Chemicals

Säkerhetsdatablad Certifikat


Image Unavailable-733-0385

qPCR low ROX-mix, ABsolute™

Beskrivning: ABsolute™ qPCR Low ROX Mixes är optimerade för användning av realtidsinstrument som med större hastighet kan detektera färger med långa våglängder, som t ex ABI 7500. Den lägre nivån av ROX underlättar normalisering över plattan, samtidigt som den bevarar analysens känslighet. Blandningarna innehåller alla komponenter som krävs för att utföra kvantitativ PCR, med undantag för templat och primrar/prober. 2X qPCR mixen innehåller reaktionsbuffert som tillhandahåller hög känslighet, specificitet och reproducerbarhet.
Artikel nummer : 733-0385
UOM: 1 * 1 KIT
Leverantör: Thermo Fisher Scientific
Product Image-MILF3096

Chemicon® BacterialXpress™ Nucleic Acid Extraction Kit

Beskrivning: BacterialXpress™ Nucleic acid extraction kit is intended for the purification of bacterial DNA from plasma, serum, cerebral spinal fluid, amniotic fluid, tissue, bone marrow, and cell culture.
Artikel nummer : MILF3096
UOM: 1 * 50 Items
Leverantör: Merck
Product Image-4028-01

Formamid ≥99.5%, Ultrapure Bioreagent for denaturing nucleic acids prior to analysis, J.T.Baker®

Beskrivning: For denaturing nucleic acids prior to analysis.
Artikel nummer : 4028-01
UOM: 1 * 500 mL
Leverantör: Avantor

Certifikat


Product Image-4056-01

Fenol ≥99% contains no preservatives, white fused crystals, Ultrapure Bioreagent for extraction of nucleic acids, J.T.Baker®

Beskrivning: Fused white crystals. For extraction of nucleic acids. Packaged under nitrogen. Lot analysis on label.
Artikel nummer : 4056-01
UOM: 1 * 500 g
Leverantör: Avantor
Product Image-ENZOENZ450150100

EpiXtract® Nuclear protein isolation kit II (nucleic acid-free)

Beskrivning: Rapid, reliable method to isolate nucleic acid-free nuclear proteins in mammalian cells and tissues.
Artikel nummer : ENZOENZ450150100
UOM: 1 * 1 KIT
Leverantör: ENZO LIFE SCIENCES
Image Unavailable-733-1975

Accessories and consumables for KingFisher™ Flex

Beskrivning: Automated nucleic acid purification system, accessory, KingFisher™ Flex 96 KF head
Artikel nummer : 733-1975
UOM: 1 * 1 Items
Leverantör: Thermo Fisher Scientific
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Aurintricarboxylic acid ≥85%, pulver, Practical grade for titration, Sigma-Aldrich®

Beskrivning: Readily polymerizes in aqueous solution, forming a stable free radical that inhibits protein-nucleic acid interactions. It is a potent inhibitor of ribonuclease and topoisomerase II by preventing the binding of the nucleic acid to the enzyme. It stimulates tyrosine phosphorylation processes including the Jak2/STAT5 pathway in NB2 lymphoma cells, ErbB4 in neuroblastoma cells, and MAP kinases, Shc proteins, phosphatidylinositide 3-kinase and phospholipase Cγ in PC12 cells. Inhibits apoptosis. It prevents down-regulation of Ca²⁺ -impermeable GluR2 receptors and inhibits calpain, a Ca²⁺ -activated protease that is activated during apoptosis.
Artikel nummer : A1895-25G
UOM: 1 * 25 g
Leverantör: SIGMA ALDRICH MICROSCOPY
Image Unavailable-PRSI33-405

Anti-IFNA2 Mouse Monoclonal Antibody [clone: N39]

Beskrivning: Recognises a protein of 16-27 kDa, identified as human interferon-II) (IFN-(II). Its epitope maps between aa112-148 of IFN-II) (total aa172). This mAb is specific for IFN-(II) and does not cross-react with IFN-(I). The site recognized by this mAb is called "site I" and is responsible for the antiviral and anti-proliferative activities of IFN-(II). Epitopes of N27 and N39 mAbs are different and represent a good combination of antibodies to set up an ELISA assay for the quantitation of IFN-(II) after viral infections. The IFN- family consists of 24 or more genes or pseudo-genes. IFN-II) is one of the two distinct families (I and II) of human IFN-. The -interferon are mainly produced by lymphocytes, monocytes, macrophages, and cell lines such as Namalwa and KG1 following induction by viruses, nucleic acids, and glucocorticoid hormones. They are involved in virus resistance on target cells, inhibition of cell proliferation, induction of cytokines and regulation of expression of MHC class I antigens.
Artikel nummer : PRSI33-405
UOM: 1 * 100 µG
Leverantör: ProSci Inc.
Image Unavailable-71978-3

One step RT-PCR master mix kit

Beskrivning: The One Step RT-PCR Master Mix Kit allows rapid, sensitive analysis of gene expression from tissues and cells. It can replace methods for detecting and quantifying gene expression such as Northern blots,<i> in situ</i> hybridisation, dot blots, S nuclease assays and conventional two step RT-PCR. The kit utilises recombinant <i>Thermus thermophilus</i> (rTth) DNA Polymerase, which acts as both a thermostable RNA dependent DNA polymerase and a DNA dependent DNA polymerase. The rTth DNA Polymerase is provided in a 2X master mix with an antibody for antibody-mediated hot start, optimised buffer, and ultrapure deoxynucleotides. Antibody-mediated Hot Start enhances specificity of both reverse transcription and PCR. The kit enables cDNA synthesis from input RNA followed by PCR amplification of the cDNA in a single reaction, with no additional hands-on requirement for buffer changes or adding reagents. Typically, detection of a specific transcript requires only 2 hours.
Artikel nummer : 71978-3
UOM: 1 * 1 KIT
Leverantör: Merck Millipore (Novagen)
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