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TNBT diformazan

Leverantör: TCI EUROPE

Beskrivning: TNBT diformazan

BCIP/TNBT alkaline phosphatase substrate

Artikel nummer : (ROCKBCBT-100)

Leverantör: Rockland Immunochemicals

Beskrivning: BCIP/TNBT Membrane Alkaline Phosphatase Substrate is an artificially manufactured chromogenic substrate for use in sensitive colorimetric assays. BCIP/TNBT can be utilized in ELISA, in situ hybridization, and IHC. Alkaline phosphatase hydrolyzes the BCIP to 5-bromo-4-chloro-3-indole, which is oxidized by oxygen in the atmosphere, yielding a blue dye. In the presence of TNBT, an insoluble dark blue precipitate is formed after a reduction reaction instead.

UOM: 1 * 100 mL

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Tetrazoliumblåklorid for colorimetric determination of reducing compounds, Sigma-Aldrich®

Leverantör: SIGMA-ALDRICH MICROSCOPY

Beskrivning: Tetrazolium blue chloride (BTC) is a colorless tetrazolium compound that upon reduction forms a colored diformazan. The reactivity has been applied to the analysis of reducing compounds including the nanomolar detection of reducing sugars and carbidopa and methyldopa. BTC has been used for histological staining of mouse coronal brain sections and rat brain sections. In combination with 4-nitrocatechol, tetrazolium blue chloride forms a ternary complex with molybdenum(VI) for extraction and spectrophotometric determination of molybdenum. It has also been used to stain Francisella tularensis cells for tularemic microagglutination test.

Nitroblå tetrazolium ≥90%, Sigma-Aldrich®

Leverantör: SIGMA-ALDRICH MICROSCOPY

Beskrivning: Nitrotetrazolium blue (NBT), in combination with BCIP (5-bromo-4-chloro-3-indolyl-phosphate), is required for the detection of labeled probes in western blots. It is part of the colorimetric detection assay. NBT and BCIP dyes detect alkaline phosphatase activity, thereby generating an insoluble purple dye (diformazan). The dye is visible at areas where the labeled probe has hybridised to its target.