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Product Image-K1642

Maxima™ First strand cDNA synthesis kit

Leverantör: Thermo Fisher Scientific
Beskrivning: The Maxima™ First Strand cDNA Synthesis Kit is a convenient system optimised for use as a first step in two step RT-qPCR. The kit includes the Maxima™ Reverse Transcriptase, which is an advanced enzyme derived by<i> in vitro</i> evolution of M-MuLV RT for RT-qPCR application. The enzyme features high thermostability, robustness and increased synthesis rates in RT-qPCR.

Product Image-28-9006-46

illustra™ Hot Start mix, Ready-To-Go™

Leverantör: Cytiva
Beskrivning: illustra™ Hot Start Mix, Ready-To-Go™ is a pre-formulated and pre-dispensed, freeze-dried PCR reagent mix, including high quality PuReTaq™ DNA polymerase and a hot start activator protein, for increased specificity and reproducibility of PCR amplifications. The Ready-To-Go™ beads are provided in either 0,5 or 0,2 ml tubes that are compatible with most thermal cyclers.

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Product Image-F-122S

Phire® Hot Start II DNA polymerase

Leverantör: FINNZYMES REAGENTS
Beskrivning: Phire® Hot Start II DNA Polymerase is faster, extremely robust, and capable of amplifying long DNA fragments with high yields. Phire® Hot Start II DNA Polymerase incorporates a dsDNA-binding domain which allows short extension times (10 to 15 s/kb), improves yields, and increases fidelity 2-fold compared to <i>Taq</i> DNA polymerase. In addition, the Hot Start technology allows complete reactivation of the enzyme in “zero-time” at standard cycling temperatures. This combination of features makes the polymerase an ideal solution for routine and high throughput PCR applications. Phire® Hot Start II DNA Polymerase delivers superior performance in conventional thermal cyclers as well as in fast instruments, such as the Piko® Thermal Cycler.

Product Image-AATB17316

ReadiLink™ iFluor® 647 FISH Fluorescence Imaging Kit

Artikel nummer : (AATB17316)
Leverantör: AAT BIOQUEST
Beskrivning: The kit provides Taq DNA polymerase enzyme which incorporates iFluor® 647-dUTPs in the target DNA through Polymerase Chain Reaction (PCR).
UOM: 1 * 25 Tests
Product Image-F-548L

Phusion® Flash high-fidelity PCR master mix

Leverantör: FINNZYMES REAGENTS
Beskrivning: Phusion® Flash High-Fidelity PCR Master Mix is a 2X master mix enabling the use of extremely short PCR protocols without compromising either the fidelity or the yield in the reaction. The master mix utilises Phusion® Flash II DNA Polymerase, a proofreading DNA polymerase modified from Phusion® Hot Start II High-Fidelity DNA Polymerase. The fidelity of the Phusion® Flash II DNA Polymerase is 25-fold greater than <i>Taq</i>.

Product Image-AATB17310

ReadiLink™ iFluor® 488 FISH Fluorescence Imaging Kit

Artikel nummer : (AATB17310)
Leverantör: AAT BIOQUEST
Beskrivning: The kit provides Taq DNA polymerase enzyme, which incorporates iFluor® 488-dUTPs in the target DNA through Polymerase Chain Reaction (PCR).
UOM: 1 * 25 Tests
Product Image-K1081

DreamTaq™ Green PCR Master Mix (2X)

Leverantör: Thermo Fisher Scientific
Beskrivning: DreamTaq Green PCR Master Mix (2X) is a ready to use solution containing DreamTaq™ DNA polymerase, optimised DreamTaq Green buffer, MgCl<sub>2</sub> and dNTPs. The master mix is supplemented with two tracking dyes and a density reagent that allows for direct loading of the PCR product on a gel. The dyes in the master mix do not interfere with PCR performance and are compatible with downstream applications, such as DNA sequencing, ligation and restriction digestion. The master mix retains all features of DreamTaq DNA polymerase. It is capable for robust amplification up to 6 kb from genomic DNA and up to 20 kb from viral DNA.

Certifikat

Product Image-D6904-04

E.Z.N.A.® Plasmid Midi kit

Leverantör: OMEGA BIO-TEK
Beskrivning: E.Z.N.A.® Plasmid Midi Kit offers a novel and reliable method of isolating up to 200 µg of plasmid DNA in less than 60 minutes. This kit combines HiBind® membrane technology with the time-tested consistency of alkaline SDS lysis of bacterial cells to deliver high-quality plasmid DNA. The DNase/RNase-free HiBind® DNA midi columns operate by eliminating time consuming phenol-chloroform extractions and alcohol precipitations. Plasmid purification using the E.Z.N.A.® Plasmid Midi Kit follows a simple bind-wash-elute procedure thereby making it possible for multiple samples to be processed. Although yields vary according to plasmid copy number, <i>E.coli </i>strain, and conditions of growth, 50 ml of an overnight culture in LB medium will typically produce 200 µg of high-copy number plasmid DNA. When working with low-copy number plasmids the starting culture volume can be increased up to 100 ml. High-quality DNA is ready for immediate use in routine molecular biology applications, such as automated fluorescent sequencing, restriction enzyme digestion, and transfection screening.

Säkerhetsdatablad

Product Image-F-549L

Phusion® Hot Start II high-fidelity DNA polymerase

Leverantör: FINNZYMES REAGENTS
Beskrivning: Phusion® Hot Start II High-Fidelity DNA Polymerase allows the use of a wide range of primers, including those with low melting temperatures. Phusion® Hot Start II High-Fidelity DNA Polymerase is ideal for PCR applications from routine PCR to highly demanding applications, such as cloning and high throughput PCR. It incorporates a unique dsDNA-binding domain making this Pyrococcus-like proofreading DNA polymerase extremely robust, rapid, and accurate.

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RevertAid™ H minus first strand cDNA synthesis kits

Leverantör: Thermo Fisher Scientific
Beskrivning: RevertAid™ H Minus First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from RNA templates. The kit includes RevertAid™ H Minus Reverse Transcriptase, which has a point mutation that completely eliminates RNase H activity. Therefore, it does not degrade RNA in RNA-DNA hybrids during synthesis of the first strand cDNA.

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Real-Time PCR kit for detection of Cronobacter sakazakii

Leverantör: Q BIOANALYTIC
Beskrivning: Cronobacter sakazakii (formerly Enterobacter sakazakii) is found naturally in the environment and can survive in dry conditions. It occurs in dry foods, such as powdered infant formula, milk powder and starches as well as in teas. Especially young infants are at high risk developing severe meningitis, if infected. All common Real-Time PCR block cyclers are usable for the test. Each reactions contains an internal amplification control. Therefore, false negative results due to inhibition of the reaction can be excluded. The test included the UNG enzyme to prevent re-amplification of contaminating amplicons. The test can also be used for the confirmation of bacterial colonies. The Real-Time PCR Test allows the detection of the species with high accuracy and is more precise than, cultural methods often are.

Product Image-F-537L

Phusion® Green Hot Start II High-Fidelity DNA Polymerase

Leverantör: FINNZYMES REAGENTS
Beskrivning: Phusion® Green Hot Start II High-Fidelity DNA Polymerase is a combination of Phusion® Hot Start II DNA Polymerase and 5× Phusion® Green buffers. The buffers include a density reagent and two tracking dyes for direct loading of PCR products on a gel. The coloured buffer does not interfere with excellent performance of Phusion® Hot Start II DNA polymerase and is compatible with downstream applications such as DNA sequencing, ligation and restriction digestion.

Image Unavailable-K1612

First strand cDNA synthesis kit

Artikel nummer : (K1612)
Leverantör: Thermo Fisher Scientific
Beskrivning: The First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from mRNA or total RNA templates. The kit uses M-MuLV Reverse Transcriptase, which has lower RNase H activity compared to AMW reverse transcriptase. The enzyme maintains activity at 37 °C and is suitable for synthesis of cDNA up to 9 kb. The recombinant Thermo Scientific RiboLock RNase Inhibitor, supplied with the kit, effectively protects RNA from degradation at temperatures up to 55 °C.
UOM: 1 * 1 KIT
Product Image-F-541

Phusion® site-directed mutagenesis kit

Artikel nummer : (F-541)
Leverantör: FINNZYMES REAGENTS
Beskrivning: Phusion® Site-Directed Mutagenesis Kit is a versatile and efficient tool for introducing point mutations, insertions, or deletions in any type of plasmid DNA.
UOM: 1 * 1 KIT
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Real-Time PCR kit for detection of Listeria monocytogenes

Leverantör: Q BIOANALYTIC
Beskrivning: OneCup it‘s so easy! The OneCup assay comes along with a minimum of pipetting steps. Everything is included in the mix. You only need to combine 20μl of the mix with 2 μl of your DNA sample and everything is ready. You have a minimum of hands-on time and you avoid mistakes during pipetting. You can run the test on all common block cyclers. Application: Detection of Listeria monocytogenes in food samples and environmental samples subsequent to a pre-enrichment step according to DIN EN ISO 20837 and 20838.Test principle. The test is dedicated to detect DNA of Listeria monocytogenes in a sample using Real-Time PCR in compliance with the ISO method mentioned below. The kit uses the TaqMan® principle. Thus the requirements of the standard, that an amplification product has to be confirmed by a hybridization step, is fulfilled. Each reactions contains an internal amplification control. Therefore, false negative results due to inhibition of the reaction can be excluded. The test included the UNG enzyme to prevent re-amplification of contaminating amplicons.

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NovaTaq PCR master mix

Artikel nummer : (71007-3)
Leverantör: Merck Millipore (Novagen)
Beskrivning: The NovaTaq PCR Master Mix is a ready to use 2X concentrated mixture of NovaTaq DNA polymerase, ultra-pure deoxynucleotides, and reaction buffer without magnesium chloride. The master mix simplifies the assembly of PCR reactions and offers advantages of times savings, consistency, and minimal risk of contamination. Simply add the NovaTaq PCR master mix to an equal volume containing the required amount of magnesium chloride, DNA template, and primers, and the reaction is ready for thermal cycling. The final diluted reaction contains 2,5 units of NovaTaq DNA polymerase per 100 µl. Sufficient components are included for 200 standard 50 µl (or 100×100 µl) amplification reactions.
UOM: 1 * 250 EEZ
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Lager för denna produkt är begränsat, men kan vara tillgänglig i ett lager nära dig. Se till att du är inloggad på hemsidan så att tillgängligt lager kan visas. Om call fortfarande visas och du behöver hjälp, ring oss på 08-621 34 20.
Lager för denna produkt är begränsat, men kan vara tillgänglig i ett lager nära dig. Se till att du är inloggad på hemsidan så att tillgängligt lager kan visas. Om call fortfarande visas och du behöver hjälp, ring oss på 08-621 34 20.
VWR International AB säljer bara etanol till skattebefriade kunder. Denna produkt ä reglerad enligt svensk lagstiftning. Om ytterligare information krävs av dig kommer du att kontaktas via e-post.
VWR International AB säljer bara etanol till skattebefriade kunder.
Denna produkt ä reglerad enligt svensk lagstiftning. Om ytterligare information krävs av dig kommer du att kontaktas via email.
Produkten har blockerats av din organisation. Vänligen kontakta din inköpsavdelning för mer information.
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Denna produkt är inte längre tillgänglig. Alternativ kan vara tillgängliga genom att söka med VWR art.nr ovan. Om du behöver ytterligare hjälp, ring VWR Kundservice på 08-621 34 20.
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