Du sökte efter: BIOWEST

Leverantör: BIOWEST

Beskrivning: Trypsin is a porcine pancreas-derived enzyme that is commonly used for the dissociation and disaggregation of anchorage-dependent mammalian cells and tissues. The concentration of trypsin necessary to dislodge the cells from their substrate depends on the sensitivity of the cells.

Säkerhetsdatablad Certifikat

Leverantör: BIOWEST

Beskrivning: Minimum Essential Medium (MEM) with Earle's Balanced Salts is a modification of Eagle's earlier medium, Basal Medium Eagle (BME), containing higher concentrations of the essential nutrients. These media have demonstrated the ability to support a variety of normal and transformed cells in culture and contain Earle's Balanced Salts, which make them suitable for use in atmospheres charged with CO₂ gas.

Säkerhetsdatablad Certifikat

Leverantör: BIOWEST

Beskrivning: Ham's F12 was originally developed for the serum-free clonal growth of Chinese Hamster Ovary (CHO) cells, lung cells and mouse L-cells. It is frequently used with dialysed serum, hormones, selenium and other supplements for serum-free cultures. It is the medium of choice for supporting the growth of cells of rodent origin, particularly rabbit and rat and has proved to be an excellent cloning medium for the culture of myeloma and hybrid cells.

Säkerhetsdatablad Certifikat

Leverantör: BIOWEST

Beskrivning: Studies to determine the nutritional requirements of many cells have been in progress since Eagle's first reports. The major essential nutrients were identified, and work became focussed on the media requirements of individual cell types. Many media designed for these purposes are now available. Among the first of these media, developed initially to study hormonal requirements of cells in culture, was a mixture of DMEM and Ham's F12 medium, known as DMEM-Ham's F12.

Säkerhetsdatablad Certifikat

Leverantör: BIOWEST

Beskrivning: RPMI are general purpose enriched media with extensive applications for a broad spectrum of mammalian and hybridoma cells, including human myeloma, mouse hybridoma, human leukocytes, and B and T lymphocytes. It was originally formulated for suspension cultures and monolayer culture of human leukaemia cells.

Säkerhetsdatablad Certifikat

Leverantör: BIOWEST

Beskrivning: These irrigating buffers and salt solutions are sterile, physiological balanced solutions intended for use in the maintenance of mammalian cells, where a chemically defined, balanced salt solution provides an environment that will maintain the structural and physiological integrity of cells <i>in vitro</i>. Applications include the intermediate steps for cultivation of cells, like washing, centrifugation, suspending and counting, as well as for many analytical methods or biochemical treatments. Principally these solutions should be used to keep the cells in suspension only for minutes or a few hours maximum. These buffers and solutions are not cell culture media.

Säkerhetsdatablad Certifikat

Leverantör: BIOWEST

Beskrivning: In 1976, Guilbert and Iscove demonstrated that precursor cells of erythrocytes and macrophages could be cultured in a reduced-serum medium supplemented with albumin, transferrin, lecithin, and selenium. Iscove's medium is a modification of Dulbecco's Modified Eagle's Medium (DME) containing selenium, additional amino acids and vitamins, sodium pyruvate, HEPES buffer, and potassium nitrate instead of ferric nitrate. Further studies have demonstrated that Iscove's medium will support murine B lymphocytes, haemopoietic tissue from bone marrow, B-cells stimulated with lipopolysaccharide, T-lymphocytes, and a variety of hybrid cells.

Säkerhetsdatablad Certifikat

Artikel nummer : (X0557-100)

Leverantör: BIOWEST

Beskrivning: Concentrated solutions of amino acids, vitamins or non essential amino acids can be added to a basal medium to enrich it and the resulting medium used as a classical MEM or BME. The final concentration of the MEM or BME must be 1X.

UOM: 1 * 100 mL

Säkerhetsdatablad Certifikat Sale

Leverantör: BIOWEST

Beskrivning: Colcemid arrests the division of cells in mitosis. It prevents the formation of the spindle apparatus responsible for cell division, thereby permitting an accumulation of metaphases.

Säkerhetsdatablad Certifikat

Leverantör: BIOWEST

Beskrivning: Amphotericin B used in tissue culture to prevent growth of yeasts and fungi. It changes the function and integrity of eucaryotic cell membranes by forming complexes with sterols (cholesterol) hence having no effect on bacteria. Cause leakage of glucose.

Säkerhetsdatablad

Artikel nummer : (X0550-100)

Leverantör: BIOWEST

Beskrivning: L (+)-glutamin 0.2 mol/l i vattenlösning

UOM: 1 * 100 mL

Säkerhetsdatablad Certifikat Sale

Artikel nummer : (L0207-500)

Leverantör: BIOWEST

Beskrivning: Schneider’s Insect Medium was developed to support the growth of excised imaginal discs from the fruit fly, <i>Drosophila melanogaster</i>. When supplemented with 5 to 20% heat-inactivated foetal bovine serum Schneider's mediuam has been found to support the rapid growth of both primary and established cultures of cells derived from Drosophila spp. and several other dipterans.

UOM: 1 * 500 mL

Säkerhetsdatablad Sale

Artikel nummer : (L0950-100)

Leverantör: BIOWEST

Beskrivning: Accustase is a ready to use cell detachment solution of proteolytic and collagenolytic enzymes. Accutase is a direct gentle replacement for trypsin solution. It can also be used on suspension cells to reduce their agglutination in order to ease counting.

UOM: 1 * 100 mL

Säkerhetsdatablad Certifikat Sale

Leverantör: BIOWEST

Beskrivning: Cell culture water is high-quality water suitable for use as a solvent in the preparation of cell culture media and laboratory reagents.

Säkerhetsdatablad Certifikat

Leverantör: BIOWEST

Beskrivning: Antibiotic-antimycotic used in tissue culture to prevent growth of bacteria (gram positive and gram negative), yeast and fungi. Penicillin acts by inhibiting bacterial cell wall synthesis.

Säkerhetsdatablad Certifikat

Artikel nummer : (L0018-100)

Leverantör: BIOWEST

Beskrivning: Penicillin-Streptomycin acts against gram-negative, gram-positive bacteria and mycoplasma.

UOM: 1 * 100 mL

Säkerhetsdatablad Certifikat Sale