Du sökte efter: RT-PCR and cDNA Reagents

Leverantör: Quantabio

Beskrivning: qScript™ cDNA-synteskit innehåller qScript™, reverstranskriptas, en blandning av MMLV, reverstranskriptas, och ribonukleashämmande protein, som optimerats för känslig och tillförlitlig cDNA-syntes med ett brett dynamiskt omfång av införd RNA. cDNA-syntesreagens finns i flera olika supermixar och kit som passar specifika tillämpningar.

Säkerhetsdatablad Certifikat

Leverantör: AGILENT

Beskrivning: Provides the highest efficiency of conversion of RNA to cDNA, fully optimised for qPCR applications.

Leverantör: ENZO LIFE SCIENCES AG

Beskrivning: AMPIGENE® 1-Step RT-PCR Kit for optimised and efficient cDNA synthesis and PCR in a single tube.

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Leverantör: ENZO LIFE SCIENCES AG

Beskrivning: AMPIGENE® cDNA Synthesis Kit for efficient and sensitive cDNA synthesis, for downstream qPCR.

New Product

Leverantör: Quantabio

Beskrivning: qScript™ XLT cDNA SuperMix is a next generation tool for first-strand cDNA synthesis, providing a highly sensitive and easy to use solution for two-step RT-PCR and RT-qPCR. qScript XLT is an engineered M-MLV reverse transcriptase with reduced RNase H activity and improved activity and stability at higher temperatures. Combined with a precise mixture of reaction components, this SuperMix enables superior results over a wide dynamic range of input RNA, with up to 8-fold higher sensitivity than cDNA synthesis kits utilising an RNase H(+) reverse transcriptase (RT).

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Artikel nummer : (733-2013)

Leverantör: Quantabio

Beskrivning: qScript™ microRNA cDNA-synteskit har utformats för att omvandla miRNA:er till cDNA med utgångspunkt från total-RNA eller RNA preparat som föranrikats på miRNA. miRNA poly(A)-denyleras inte i naturen. Med qScript™ microRNA cDNA-synteskitet polyadenyleras miRNA:er i en poly(A)-polymerasreaktion. qScript™ omvänt transkriptas och andra nödvändiga reagenser för cDNA-syntes tillförs sedan för att omvandla de poly(A)-svansförsedda microRNA:erna till cDNA med hjälp av en oligo-dT-adapterprimer. Adapterprimern har en unik sekvens vid 5’-änden som tillåter amplifiering av cDNA i realtids-qRT-PCR-reaktioner.

UOM: 1 * 25 Assays

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Leverantör: Quantabio

Beskrivning: Faster, better, tougher next generation cDNA SuperMix for cDNA synthesis.

Leverantör: Quantabio

Beskrivning: The qScript™ XLT One-Step RT-PCR Kit is a convenient and highly sensitive system for amplification of RNA templates up to 2 kb. cDNA synthesis and PCR amplification are carried out in the same tube without opening between procedures. This system has been optimised to deliver maximum endpoint RT-PCR efficiency, sensitivity, and specificity.

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Leverantör: Thermo Fisher Scientific

Beskrivning: Thermo Scientific™ Maxima™ H Minus cDNA Synthesis Master Mix with dsDNase provides a simple workflow that combines genomic DNA elimination and cDNA synthesis in a one-tube procedure. The cDNA reaction components are pre-mixed into a complete master mix that is convenient to use, reduces pipetting steps, and is optimised for cDNA synthesis in two-step quantitative RT-PCR (RT-qPCR) applications. Maxima™ H Minus cDNA Synthesis Master Mix is optimised for reproducible cDNA synthesis at elevated temperatures (50 to 65°C) within 30 minutes.

Leverantör: Thermo Fisher Scientific

Beskrivning: Maxima™ Reverse Transcriptase is an advanced enzyme derived by <i>in vitro</i> evolution of M-MuLV RT for RT-qPCR application. The enzyme features high thermostability, robustness and increased synthesis rates in RT-qPCR. The Maxima™ Reverse Transcriptase is capable of cDNA synthesis at elevated temperatures (50 to 60 °C) and completion of the reverse transcription reaction in 15 to 30 minutes. The use of this enzyme in combination with Maxima™ qPCR Master Mixes ensures high specificity, sensitivity and wide linear range of two step RT-qPCR. Applications include first strand cDNA synthesis, RT-PCR, and RT-qPCR.

Leverantör: Thermo Fisher Scientific

Beskrivning: RevertAid™ H Minus Reverse Transcriptase (RT) is a genetically modified M-MuLV RT. It differs from the M-MuLV RT by its structure and catalytic properties. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity, but lacks RNase H activity due to point mutation in the RNase H domain. RevertAid™ H Minus Reverse Transcriptase does not degrade RNA in RNA-DNA hybrids during synthesis of the first strand cDNA and therefore high yields of full-length cDNA from long templates are obtained. RevertAid™ H Minus Reverse Transcriptase maintains activity over a wide temperature range (42 to 55 °C), which makes the enzyme an ideal tool for reverse transcription of RNAs having a high degree of secondary structure.

Leverantör: Thermo Fisher Scientific

Beskrivning: The RevertAid™ First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from RNA templates. The kit is suitable for synthesis of cDNA up to 13 kb. The kit uses RevertAid™ Reverse Transcriptase which has lower RNase H activity compared to AMV reverse transcriptase. The recombinant RiboLock™ RNase Inhibitor, supplied with the kit, effectively protects RNA template from degradation. It is fully compatible with reverse transcription reaction, as it maintains activity at temperatures up to 55 °C.

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Leverantör: Thermo Fisher Scientific

Beskrivning: The Maxima™ First Strand cDNA Synthesis Kit is a convenient system optimised for use as a first step in two step RT-qPCR. The kit includes the Maxima™ Reverse Transcriptase, which is an advanced enzyme derived by<i> in vitro</i> evolution of M-MuLV RT for RT-qPCR application. The enzyme features high thermostability, robustness and increased synthesis rates in RT-qPCR.

Leverantör: Thermo Fisher Scientific

Beskrivning: Thermo Scientific™ Maxima™ H Minus cDNA Synthesis Master Mix provides all cDNA synthesis reaction components in a convenient one-tube master mix. It is optimised for highly efficient cDNA synthesis for two-step quantitative RT-PCR (RT-qPCR) applications.

Artikel nummer : (K1612)

Leverantör: Thermo Fisher Scientific

Beskrivning: The First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from mRNA or total RNA templates. The kit uses M-MuLV Reverse Transcriptase, which has lower RNase H activity compared to AMW reverse transcriptase. The enzyme maintains activity at 37 °C and is suitable for synthesis of cDNA up to 9 kb. The recombinant Thermo Scientific RiboLock RNase Inhibitor, supplied with the kit, effectively protects RNA from degradation at temperatures up to 55 °C.

UOM: 1 * 1 KIT

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Leverantör: Thermo Fisher Scientific

Beskrivning: Maxima™ H Minus First Strand cDNA Synthesis Kit is a complete system for highly efficient synthesis of first strand cDNA. The kit uses the Maxima™ H Minus Reverse Transcriptase (RT), which is an advanced enzyme derived by<i> in vitro</i> evolution of M-MuLV RT. The enzyme features the highest thermostability among the derivatives of M-MuLV RT and lacks RNase H activity. The Maxima™ H Minus First Strand cDNA Synthesis Kit allows synthesis of long cDNAs up to 20 kb at elevated temperatures (up to 65 °C), superseding other systems' abilities to produce full-length cDNA. Due to increased synthesis rates the reaction can be completed in 30 minutes.