Ponceau S, Bioreagent för elektrofores, Sigma-Aldrich®
Leverantör: SIGMA-ALDRICH MICROSCOPY
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Varning
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Synonyms:
3-Hydroxy-4-(2-sulpho-4-[4-sulphophenylazo]phenylazo)-2,7-naphthalenedisulphonic acid sodium salt, CI 27195, Ponceau, 3-Hydroxy-4-((2-sulpho-4-((4-sulphophenyl)diazenyl)phenyl)diazenyl)naphthalene-2,7-disulphonic acid, tetrasodium salt
M-Clarity™ quality level = MQ200
Ponceau S is the sodium salt of a diazo dye. It is used to make a stain for rapid reversible staining of protein bands on nitrocellulose or PVDF membranes (Western blots) and also for staining protein on cellulose acetate membranes. The stain is reversed with water washes and aids in subsequent immunological detection. The following are two common stain formulations: 0.1% Ponceau S (w/v) in 5% acetic acid 2% Ponceau S (w/v) in 30% TCA, 30% sulfosalicylic acid.
- Ponceau S has been used for membrane staining (Western blotting)
Formel:
C₂₂H₁₂N₄Na₄O₁₃S₄ MW: 760,56 g/mol Storage Temperature: Room Temperature |
MDL Number:
MFCD00003892 CAS nummer: 6226-79-5 EINECS: 228-319-2 |
Specifikation testresultat
Infrared spectrum | Conforms to Structure |
Solubility (Turbidity) | Clear |
Solubility (Colour) c = 4mg/4ml; Water |
Red to Very Dark Red |
Suitability Suitable for Use as a Stain in Electrophoresis |
Suitable |
Wavelength C = 0.015 G/L, Di water |
518 - 522 nm |
Extinction Coefficient | ≥33000 |
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