5-Fluoroorotic acid ≥98%, pale yellow crystalline powder
Leverantör: MP Biomedicals
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Varning
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Synonyms:
5-FOA
FOA is a derivative of a pyrimidine precursor and is selectively toxic to yeast cells which synthesize orotidine-5’-phosphate decarboxylase. FOA has been used in a positive selection for uracil-requiring mutants(in the presence of large numbers of wild-type cells) lacking OMPdecase or OPRTase activities. This results in efficient strains for effective transformation systems.
5-Fluoroorotic acid is useful in the selection of orotidine-5′-phosphate decarboxylase mutants of Saccharomyces cerevisiae.It has been used as a selective agent in yeast molecular genetics.It has also been used in the positive selection for uracil auxotrophs of the sulphur-dependent thermophilic archaebacterium sulpholobus acidocaldarius.
Soluble in hot water or methanol; soluble in 1:1 NH4OH : H2O (50 mg/ml with gentle heating).
Formel:
C₅H₃FN₂O₄ MW: 174,09 g/mol Smältpunkt: 278 °C (dec., lit.) |
MDL Number:
MFCD00042526 CAS nummer: 703-95-7 EINECS: 211-876-0 |
Specifikation testresultat
Identity Test | Passes |
Purity | ≥98% |
FTIR | Conforms to Reference |
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