5-Fluoroorotic acid enzyme substrate
Leverantör: Thermo Fisher Scientific
Synonyms:
5-FOA
Thermo Scientific 5-Fluoroorotic Acid (5-fluorouracil-6-carboxylic acid monohydrate; 5-FOA) is used in yeast molecular genetics to detect expression of the URA3 gene, which encodes orotine-5'-monophosphate (OMP) dicarboxylase
Yeast strains carrying a mutation in the URA3 gene grow in the presence of 5-FOA if the media is supplemented with uracil. 5-FOA is frequently used in experiments with Saccharomyces cerevisiae (URA3), Schizosaccharomyces pombe (URA4 and URA5), Candida albicans (URA3), and E. coli (pyrF).
Yeasts with an active URA3 gene (Ura+) convert 5-FOA to fluorodeoxyuridine, which is toxic to cells
Formel:
C₅H₃FN₂O₄ MW: 174,09 g/mol |
MDL Number:
MFCD00042526 CAS nummer: 703-95-7 EINECS: 211-876-0 |
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