Serum replacement, Corning® Nu-Serum™ IV
Leverantör: Corning
Corning® Nu-Serum™ IV odlingsmediatillsats ger ett låg-proteinalternativ till nyfödd kalv, fetalbovin och andra sera som rutinanvänds för cellodling.
The low-protein content facilitates protein purification, virus production (Okada, M., et al., Microbiological Immunol.).
Tested at a concentration of 10% for the ability to stimulate a ≧100-fold increase in growth of BHK-21 and a ≧13-fold increase in growth of BALB/c-3T3 cells. Tested for total protein content, pH, and osmolarity (information included with product). Filtered (0.2 µm membrane) and tested and found negative for the presence of bacteria, fungi, mycoplasma, and viruses (bovine diarrhea virus, bovine parvovirus, bovine adenovirus, reovirus, and rabies virus); also tested for the absence of cytopathic effects (CPE), inclusion bodies and hemadsorption. Tested for endotoxin (LAL assay).
31:657, 1987), monoclonal antibody production and screening (Sjogren-Janssen, E., e al., J. Immunol. Meth. 84:357, 1985), and increases the frequency of successful transfection of cells (Seed, B., et al., Proc. Natl. Acad. Sci. USA 84:3365, 1987; Duval, N., et al., J. Cell Biol. 118:641, 1992.
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