3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) ≥98%, yellow powder cell culture reagent
Leverantör: MP Biomedicals
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Varning
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Synonyms:
Thiazolyl blue tetrazolium bromide, MTT, 2-(4,5-Dimethylthiazol-2-yl)-3,5-diphenyl-2H-tetrazol-3-ium bromide
Storage: +4°C, protect from light
3- (4,5-Dimethyl-2-thiazolyl-2)-2,5-diphenyl-tetrazolium bromide is used as a colorimetric metabolic activity indicator in cell viability assays. Thiazolyl Blue Tetrazolium Blue (MTT) is a dye used in measurement of cell proliferation. MTT produces a yellowish solution that is converted to dark blue, water-insoluble MTT formazan by mitochondrial dehydrogenases of living cells. The blue crystals are solubilized with acidified isopropanol and the intensity is measured colorimetrically at 570 nm. MTT has been used as a histochemical/cytochemical reagent and for the detection of NAD. ADP-linked enzyme systems in tissue cannot be detected with MTT, due to binding of the cation by the cyanide trap used. MTT is rapidly reduced to the formazan, which chelates with nickel, copper, and cobalt; the cobalt chelate has been used in oxidative systems.
- Cell Culture Reagent Grade
- Presentation: Yellow Powder
- Soluble in methyl alcohol (heat), methanol (10 mg/mL with heat - clear, yellow solution)and water (5 mg/mL-clear to very slightly hazy, yellow to bright yellow solution).
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Formel:
C₁₈H₁₆N₅BrS MW: 414,33 g/mol Smältpunkt: 189…199 °C Storage Temperature: Kylskåp |
MDL Number:
MFCD00011964 CAS nummer: 298-93-1 EINECS: 206-069-5 |
Specifikation testresultat
| Identity Test | Passes |
| Purity | ~98% |
| FTIR | Conforms to Standard |
| Melting Point | 189 - 199°C |
| UV/Vis: I max (methanol) | 376 ± 2 nm; 242 ± 2 nm |
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