Lugol's iodine solution, Sigma-Aldrich®
Leverantör: Merck
M-Clarity™ quality level = MQ300
Diluted iodine-potassium iodide solution used to form a crystal violet dye-iodine complex in Gram staining.
In bacteriology, the Gram staining allows a fast differentiation of bacteria in Gram-positive and Gram-negative. The mureine structure of the bacteria walls is the basis of the color affinity. Bacteria will be stained with Gram's crystal violet solution - an aniline dye - in the first step. After the treatment with Gram's iod solution (Lugol’s solution), a dye-iod complex will form. During the decolorizing step, this complex stays in the multilayer mureine structures of the Gram-positive bacteria and they will appear blue. Gram-negative bacteria have a monolayer mureine structure only, the dye-iod complex does not stay bound to the cellwall, they will be decolorized . Gram-negative bacteria will be counterstained by Gram's safranin solution and appear orange. A 500 mL PE-bottle of Gram's Lugol's solution is sufficient for staining up to 250 slides. Lugol’s solution is also a part of the Gram-color stain set. It is an IVD & CE product.
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Specifikation testresultat
| Suitability for microscopy | passes test |
| gram-positve microorganisms | blue-violet |
| gram-negative microorganisms | pink to red |
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