Coomassie® Brilliant Blue R-250 ~50%, Technical Grade, Sigma-Aldrich®
Leverantör: SIGMA-ALDRICH MICROSCOPY
Synonyms:
Serva Blue R, Brillant blue R, N-[4-[[4-[(4-Ethoxyphenyl)amino]phenyl][4-[ethyl[(3-sulphophenyl)methyl]amino]phenyl]methylene]-2,5-cyclohexadien-1-ylidene]-N-ethyl-3-sulpho-benzenemethanaminium inner salt, sodium salt (1:1), Coomassie Blue R 250, Brilliant Cyanine 6B, Hydrogen [4-[4-(p-ethoxyanilino)-4'-[ethyl(m-sulphonatobenzyl)amino]benzhydrylene]cyclohexa-2,5-dien-1-ylidene](ethyl)(m-sulphonatobenzyl)ammonium monosodium salt, Acid Blue 83, Brilliant Blue R250, Brilliant Blue R, Brilliant indocyanine 6B
M-Clarity™ quality level = MQ200
Brilliant Blue R (C.l. 42660) is a triaminotriarylmethane dye with two amino groups being benzylated and one phenylated. It is also known as brilliant indocyanine 6B and coomassie blue R250. Brilliant Blue R has been widely used for protein band detection after SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis).
- Its other applications include:
- as a protein stain on epoxy-embedded tissue sections
- for quantitative micro densitometry of protein in sections
- for staining chromatids, actinin microfilaments, developing neurites, and sperm acrosomes
- For detection of protein bands following electrophoresis
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Formel:
C₄₅H₄₄N₃NaO₇S₂ MW: 825,98 g/mol Smältpunkt: 174…180 °C Storage Temperature: Room Temperature |
MDL Number:
MFCD00041762 CAS nummer: 6104-59-2 EINECS: 228-060-5 |
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Specifikation testresultat
| Appearance (Form) | Powder |
| Suitability For Detection of Protein Bands Following Electrophoresis | Suitable |
| Solubility (Colour) | Very Dark Purple to Very Dark Blue and Dark Blue-Black |
| Carbon | 32.0 - 42.0 % |
| Nitrogen | 2.5 - 3.5 % |
| Dye Content | Approximately 50% |
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