Rhodamine 123 fluorescent dye, mitochondria dye
Leverantör: SIGMA-ALDRICH MICROSCOPY
Synonyms:
3,6-Diamino-9-(2-(methoxycarbonyl)phenyl xanthylium chloride, 3,6-Diamino-9-(2-(methoxycarbonyl)phenyl)xanthylium chloride, 2-(6-Amino-3-imino-3H-xanthen-9-yl)benzoic acid methyl ester
M-Clarity™ quality level = MQ100
Rhodamine 123 is useful as a laser dye and to have selective cell growth effects. It has also been used to measure mitochondrial membrane potential.
Rhodamine 123 is a fluorescent dye most commonly used in flow cytometry as functional reporter for P-glycoprotein (Pgp). It is part of the rhodamine family of flurone dyes. The dye is mostly used to assess mitochondrial bioenergetics in living cells. Functional assays for multi-drug resistance (MDR) proteins are better prognostic indicators in cancer therapy than levels of MDR protein expression. Rh 123 can be used in multiparameter analyses without fluorescence interference in combination with common protein labeling dyes such as PE-Cy5 and AMCA (7-amino-4-methylcoumarin-3-acetic acid). Recent reports indicate Rh 123 may also be a substrate of multi-drug resistance protein-1 (MRP1).
Fluorescent dye most commonly used in flow cytometry as functional reporter for Pgp. Functional assays for MDR proteins are better prognostic indicators in cancer therapy than levels of MDR protein expression. Rh 123 can be used in multiparameter analyses without fluorescence interference in combination with common protein labeling dyes such as PE-Cy5 and AMCA (7-amino-4-methylcoumarin-3-acetic acid). Recent reports indicate Rh 123 may also be a substrate of MRP1.
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Formel:
C₂₁H₁₇ClN₂O₃ MW: 380,82 g/mol Storage Temperature: Room Temperature |
MDL Number:
MFCD00012664 CAS nummer: 62669-70-9 |
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Specifikation testresultat
| Appearance (Form) | Powder |
| Infrared spectrum | Conforms to Structure |
| Carbon (dry basis) | 62.2 - 70.2 % |
| Nitrogen (dry basis) | 6.9 - 7.8 % |
| Water (by Karl Fischer) | ≤10.0 % |
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Solubility (Turbidity) 1mg/ml in Ethanol |
Clear to Slightly Hazy |
| Solubility (Colour) |
Conforms to Requirements Yellow-Orange |
| Recommended Retest Period | 4 Years |
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