Gram′s safranin solution for Gram staining, för mikroskopi, Sigma-Aldrich®
Leverantör: SIGMA-ALDRICH MICROSCOPY
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Varning
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Synonyms:
Safranin T solution
M-Clarity™ quality level = MQ100
Gram’s safranin solution, also known as Safranin T or Basic Red 2, is a ready-to-use staining solution that comprises 10% safranin in ethanol. Safranin is a basic, cationic, azine dye containing a planar, diaminophenazine chromophore, with a phenyl substituent attached to a ring nitrogen. The trimethyl cationic form of safranine is lipophilic, whereas the dimethyl forms are weakly hydrophilic. It is most notably used in bacteriology as a counterstain in Gram staining to differentiate between Gram-positive and Gram-negative bacteria.
- Ready-to-use solution
- Modified and designed in such a way that staining can be carried out in staining cells, on the staining rack, and in automated staining systems
Gram’s safranin solution has been used in the following applications:
as a non-toxic replacement for crystal violet for the quantification of biofilm formation
to develop a hyaluronic acid visualisation method
to study improved β-lactam susceptibility against biofilm-embedded staphylococcus aureus by 2-aminothiazole
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Formel:
C₂₀H₁₉ClN₄ MW: 350,85 g/mol Densitet: 0,986 g/ml at 20 °C Flampunkt: 46 °C Storage Temperature: Room Temperature |
MDL Number:
MFCD00011759 CAS nummer: 477-73-6 |
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Specifikation testresultat
| Appearance (colour) | Red to very dark red |
| Appearance (form) | Liquid |
| Thin layer chromatogr. | Corresponds to standard |
| Density D20/4 | 0.984 - 0.988 |
| Refractive index N20/D | 1.338 - 1.340 |
| Wavelength (1) (UV) | 525 - 535 nm |
| Extinction (1%/1 cm) (1) (UV) | Report result |
| Solvent (UV) | Ethanol 50% |
| Expiry period | 36 months |
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