Sulpho-SMCC (3-Sulpho-N-succinimidyl-4-(N-maleimidomethyl)cyclohexane-1-carboxylate sodium salt), Premium Grade, Pierce™
Leverantör: Thermo Fisher Scientific
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Varning
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Synonyms:
Sodium 1-((4-((2,5-dioxo-2,5-dihydro-1H-pyrrol-1-yl)methyl)cyclohexane-1-carbonyl)oxy)-2,5-dioxopyrrolidine-3-sulphonate, Sulphosuccinimidyl-trans-4-(N-maleimidomethyl)cyclohexane-1-carboxylate, Sulpho-SMCC Sodium Salt, SMCC, Water-Soluble, 4-(N-Maleimidomethyl)cyclohexane-1-carboxylic acid 3-sulpho-N-hydroxysuccinimide ester sodium salt, sulpho-SMCC, Sulfo-SMCC
Thermo Scientific Pierce Premium Grade Sulfo-SMCC is our highest quality formulation of amine-to-sulfhydryl crosslinker, specially characterized for applications where product integrity and risk minimization are paramount.
- Sulfo-SMCC—a water-soluble amine-to-sulfhydryl crosslinker with a stabilizing cyclohexane spacer arm
- High quality—identity and purity confirmed by several tests, including quantitative NMR
- Product integrity—enhanced level of testing and characterization compared to standard grade
- Lot retention—ample supply of past lots retained to ensure future process testing
- Change management—Change Control Notification (CCN) service
- Consistent manufacture—batch-specific manufacturing documentation review
Compared to the standard grade product, Premium Grade Sulfo-SMCC provides more clearly defined quality and product support by including (a) increased analytical testing and product characterization, (b) greater batch-specific information and quality assurance review, (c) extensive lot sample retention and (d) change control notification. Sulfo-SMCC is a water-soluble, non-cleavable and membrane-impermeable amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a medium-length cyclohexane spacer arm (8.3 angstroms). NHS esters react with primary amines at pH 7-9 to form stable amide bonds. Maleimides react with sulfhydryl groups at pH 6.5-7.5 to form stable thioether bonds. The maleimide groups of Sulfo-SMCC are unusually stable up to pH 7.5 because of the cyclohexane bridge in the spacer arm. Because it contains the hydrophilic sulfonyl moiety, Sulfo-SMCC is soluble up to nearly 10 mM in water and many commonly used buffers, thus avoiding the use of organic solvents which may perturb protein structure.
Observera: For Research Use Only. Not for use in diagnostic procedures.
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Formel:
C₁₆H₁₈N₂O₉S MW: 436,37 g/mol Storage Temperature: Frys |
MDL Number:
MFCD00054978 CAS nummer: 92921-24-9 |
Specifikation testresultat
| Product Line | Pierce™ |
| Labeling Method | Chemical Labeling |
| Crosslinker Type | Heterobifunctional |
| Reactive Moiety | Maleimide, Sulfo-NHS Ester |
| Spacer | Short |
| Form | Powder |
| Quantity | 100 mg/1 g |
| Type | Crosslinker |
| Solubility | Water |
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