BS3 (Bis(sulphosuccinimidyl) suberate sodium salt) ≥93%, Pierce™
Leverantör: Thermo Fisher Scientific
Synonyms:
BS3
Thermo Scientific Pierce BS3 (Sulfo-DSS) is bis(sulfosuccinimidyl)suberate, an amine-to-amine crosslinker that is homobifunctional, water-soluble, non-cleavable and membrane impermeable.
- Reactive groups: sulfo-NHS ester (both ends)
- Reactive towards: amino groups (primary amines)
- Amine-reactive Sulfo-NHS ester reacts rapidly with any primary amine-containing molecule
- Water-soluble; compare with DSS
- Membrane-impermeable, allowing for cell surface labeling
- High-purity, crystalline reagent can be used to create high-purity crosslinked conjugates
BS3 contains an amine-reactive N-hydroxysulfosuccinimide (NHS) ester at each end of an 8-carbon spacer arm. NHS esters react with primary amines at pH 7-9 to form stable amide bonds, along with release of the N-hydroxysulfosuccinimide leaving group. Proteins, including antibodies, generally have several primary amines in the side chain of lysine (K) residues and the N-terminus of each polypeptide that are available as targets for NHS-ester crosslinking reagents.
Because it contains the hydrophilic sulfonyl moiety, BS3 crosslinker is soluble up to ∼100 mM in water and many commonly used buffers, thus avoiding the use of organic solvents which may perturb protein structure. DSS, the non-water soluble analog of BS3 is also available for applications that require a less hydrophilic crosslinker (e.g., to effect intracellular crosslinking). DSS and BS3 have essentially identical crosslinking activity toward primary amines.
Observera: For Research Use Only. Not for use in diagnostic procedures.
Formel:
C₁₆H₁₈N₂Na₂O₁₄S₂ MW: 572,43 g/mol Storage Temperature: Kylskåp |
MDL Number:
MFCD00054982 CAS nummer: 82436-77-9 |
Specifikation testresultat
Description | Pierce BS3 (Sulfo-DSS) |
Product Type | Crosslinker |
Reactivity | Sulfo-NHS Ester |
Solubility | Water |
Product Line | Pierce™ |
Form | Powder |
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