Sulpho-NHS Biotin, EZ-Link™ No-Weigh™ Format
Leverantör: Thermo Fisher Scientific
Synonyms:
Biotin 3-sulpho-N-hydroxysuccinimide ester sodium salt, Sulphosuccinimidyl biotin
Thermo Scientific EZ-Link Sulfo-NHS-Biotin is a short-chain, water-soluble biotinylation reagent for labeling antibodies, proteins and other molecules that have primary amines.
- Protein labeling—biotinylate antibodies to facilitate immobilization, purification or detection using streptavidin resins or probes
- Cell surface labeling—biotinylates only surface proteins of whole cells because the negatively charged reagent does not permeate cell membranes
- Amine-reactive—reacts with primary amines (-NH2), such as lysine side-chains or the amino-termini of polypeptides
- Soluble—charged sulfo-NHS group increases reagent water solubility compared to ordinary NHS-ester compounds
- Irreversible—forms permanent amide bonds; spacer arm cannot be cleaved
- Very short—spacer arm (total length added to target) is 13.5 angstroms; it consists of the native biotin valeric acid group only.
Sulfo-NHS-Biotin is the shortest of three very similar EZ-Link Reagents that are water-soluble, non-cleavable, and enable simple and efficient biotinylation of antibodies, proteins and any other primary amine-containing macromolecules in solution. Specific labeling of cell surface proteins is another common application for these uniquely water-soluble and membrane impermeable reagents. Differing only in their spacer arm lengths, the three Sulfo-NHS-ester reagents offer the possibility of optimizing labeling and detection experiments where steric hindrance of biotin binding is an important factor. Sulfo-NHS-Biotin is offered in several package sizes and as complete protein labeling kits.
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Formel:
C₁₄H₁₈N₃NaO₈S₂ MW: 443,421 g/mol Storage Temperature: Frys |
MDL Number:
MFCD00078535 CAS nummer: 119616-38-5 |
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