Basic Violet 3 ethanolic aqueous solution primary stain for Gram staining, Sigma-Aldrich®
Leverantör: Merck
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Varning
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Synonyms:
4-[4,4'-Bis(dimetylamino) benzhydryliden]cyklohexa-2,5-dien-1-ylidendimetylammoniumklorid, CI 42555
The Gram staining allows a fast differentiation of bacteria in Gram-positive and Gram-negative.
The mureine structure of the bacteria walls is the basis of the color affinity. Bacteria will be stained with Gram's crystal violet solution - an aniline dye - in the first step. After the treatment with iod solution (Lugol’s solution), a dye-iod complex will form. During the decolorizing step, this complex stays in the multilayer mureine structures of the Gram-positive bacteria and they will appear blue. Gram-negative bacteria have a monolayer mureine structure only, the dye-iod complex does not stay bound to the cellwall, they will be decolorized . Gram-negative bacteria will be counterstained by safranin solution and appear orange.
Formel:
C₂₅H₃₀ClN₃ MW: 407,99 g/mol Storage Temperature: Omgivande |
MDL Number:
MFCD00011750 CAS nummer: 548-62-9 UN: 1170 ADR: 3,III |
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