Indo-1 AM ≥96% fluorescent indicator, for calcium determination
Leverantör: Biotium
Synonyms:
1-[2-Amino-5-(6-carboxyindol-2-yl)phenoxy]-2-(2'-amino-5'-methylphenoxy)ethane-N,N,N',N'-tetraacetic acid, pentaacetoxymethylester, Indo-1-(acetoxymethyl) ester, 4-(6-Carboxy-2-indolyl)-4′-methyl-2,2′-(ethylenedioxy)dianiline-N,N,N′,N′-tetraacetic acid tetrakis(acetoxymethyl) ester, Indo-1 AM Ester
Indo-1, AM ester is membrane-permeant and thus can be loaded into cells via incubation. Because of the relatively low water solubility of the AM ester, Pluronic F-127, a mild detergent, is often used as a dispersing agent to facilitate cell loading. Indo-1-AM Ester itself does not bind Ca2+ , but it is readily hydrolyzed to indo-1 by endogenous esterases once it enters cells.
- Absorption/Emission 356 nm/478 nm (no Ca2+); 330 nm/400 nm (high Ca2+)
- Extinction Coefficient 33,000 M -1 cm -1 (no Ca2+); 33,000 M -1 cm -1 (high Ca2+)
- Cell permeability: Membrane permeant
- Light yellow solid soluble in DMSO
- Store desiccated at -20°C and protect from light, especially in solution
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Formel:
C₄₇H₅₁N₃O₂₂ MW: 1009,91 g/mol Storage Temperature: Frys |
MDL Number:
MFCD00036955 CAS nummer: 112926-02-0 |
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