Urea ≥98%, white powder för molekylärbiologi
Leverantör: MP Biomedicals
Synonyms:
Carbonyl diamide, Carbamide, Diaminomethanone, Carbonyldiamide
Applications:
Urea is used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies and in the extraction of the mitochondrial complex. It is commonly used to solubilize and denature proteins for denaturing isoelectric focusing and two-dimensional electrophoresis and in acetic acid-urea PAGE gels. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form. Urea is used in cell or tissue culture media to increase the osmolality. Urea has also been used as fertilizer because of the easy availability of nitrogen; in animal feeds; it is reacted with aldehydes to make resins and plastics; condensed with malonic ester to form barbituric acid; used in the paper industry to soften cellulose.
Biochem/physiol Actions:
Urea has been shown to act as an aldosterone antagonist in the development of peanut agglutinin binding in cultured embryonic renal collecting duct epithelial cells. Urea is the principal end product of nitrogen metabolism in most mammals, formed by the enzymatic reactions of the Kreb's cycle.
Typical Working Concentration:
The use of 2 g/L urea in the culture of Kluyveromyces marxianus to produce a thermostable extracellular lipase has been described. Urea is typically used at a concentration of 8 M for protein denaturation or solubilization. A final concentration of 5 M urea is commonly used in molecular biology for sequencing gels. To prevent carbamylation, do not heat urea containing buffers above 37 °C
- Molecular Biology Reagent Grade
- Presentation: White Powder
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Formel:
NH₂CONH₂ MW: 60,06 g/mol Kokpunkt: 197 °C (1013 hPa) Smältpunkt: 132,7 °C Densitet: 1,34 g/cm³ (20 °C) Storage Temperature: Omgivande |
MDL Number:
MFCD00008022 CAS nummer: 57-13-6 EINECS: 200-315-5 REACH: 01-2119463277-33 Merck Index: 13,09935 |
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Specifikation testresultat
| Identity Test | Passes |
| Purity | >98% |
| FTIR | Conforms to Standard |
| Melting Point | 130 - 140°C |
| OD260 nm (7 M aq soln) | <0.2 |
| OD280 (7M aq soln) | <0.2 |
| pH (7 M aq soln) | 7.5 - 9.5 |
| PB | <2 ppm |
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