VWR®, Taq DNA polymerase glycerol free
Leverantör: VWR Chemicals
VWR® Taq DNA Polymerase Glycerol Free 5U/µl is a thermostable recombinant DNA polymerase, which exhibits very high activity in primer extension and other molecular biology applications. The enzyme is isolated from Thermus aquaticus and has a molecular weight of approximately 94 kDa.
- Most suitable choice for routine applications where high fidelity is not required
- High performance, thermostable DNA polymerase
- Optimal for TA cloning
VWR® Taq DNA Polymerase Glycerol Free has both a 5'→3' DNA polymerase and a 5'→3' exonuclease activity. The enzyme lacks a 3'→5' exonuclease activity (no proofreading ability). VWR® Taq DNA Polymerase Glycerol Free leaves an A' overhang, which makes the enzyme ideal for TA cloning.
10X Key Buffer: Tris-HCL pH 8,5, (NH₄)₂SO₂, 15 mM MgCl₂, 1% Tween-20®
10X Extra Buffer: 100 mM Tris-HCL pH 8,3, 500 mM KCl, 15 mM MgCl₂, 1% Triton X-100
10X Mg-Free Key Buffer: Tris-HCL pH 8,5, (NH₄)₂SO₂, 1% Tween-20®
10X Mg-Free Extra Buffer: Tris-HCL pH 8,3, KCl, 1% Triton X-100
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