Reactive quencher dyes

Leverantör: Biotium

91051 80501 80500 80502
91051.EA 8700 SEK
91051. 80501. 80500. BTIU80502
Reactive quencher dyes
System för proteinlänkning och märkning

Reactive quenchers that act as acceptors in fluorescence resonance energy transfer (FRET) applications.


  • Thiol- and amine-reactive quenchers
  • One Sample Fluorescence Resonance Energy Transfer (OS-FRET) and FRET quenchers


NFQ1, a non-fluorescent quencher, is used for the One Sample Fluorescence Resonance Energy Transfer (OS-FRET) method employing a non-fluorescent methanethiosulfonate-linked acceptor that can be reversibly coupled to a target sulfhydryl residue via a disulfide bond. After the quenched donor emission is quantitated the acceptor is removed by reduction, enabling measurement of unquenched donor emission in the same sample.


The thiol-reactive quencher, CFQ520 maleimide, has a broad and intense absorption (approximately 520 nm) but no fluorescence, making it useful as an acceptor in fluorescence resonance energy transfer (FRET) applications.


The amine-reactive quencher, CFQ520 succinimidyl ester, has a broad and intense absorption (approximately 520 nm) but no fluorescence, making it useful as an acceptor in fluorescence resonance energy transfer (FRET) applications.


N-(6-Aminohexyl)maleimide is a cross-linking reagent that can react with an amine-reactive group and a thiol group.

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